Western Blot (WB)
(Figure 1. Western blot analysis of Cyt 19/As3mt using anti-Cyt 19/As3mt antibody (MBS1753688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse heart tissue lysatesLane 3: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cyt 19/As3mt antigen affinity purified polyclonal antibody (Catalog # MBS1753688) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cyt 19/As3mt at approximately 44KD. The expected band size for Cyt 19/As3mt is at 42KD. )
Rabbit anti-Mouse Cyt 19/As3mt Polyclonal Antibody | anti-As3mt antibody
Anti-Cyt 19/As3mt Antibody
Gene Names
As3mt; Cyt19; 2310045H08Rik
Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
Cyt 19/As3mt; Polyclonal Antibody; Anti-Cyt 19/As3mt Antibody; Arsenite methyltransferase; Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III methyltransferase; As3mt; Cyt19; arsenite methyltransferase; anti-As3mt antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Cyt 19/As3mt detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-As3mt antibody
Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
Immunogen
E Coli-derived mouse Cyt 19/As3mt recombinant protein (Position: M1-D346).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Cyt 19/As3mt using anti-Cyt 19/As3mt antibody (MBS1753688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse heart tissue lysatesLane 3: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cyt 19/As3mt antigen affinity purified polyclonal antibody (Catalog # MBS1753688) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cyt 19/As3mt at approximately 44KD. The expected band size for Cyt 19/As3mt is at 42KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Cyt 19/As3mt using anti-Cyt 19/As3mt antibody (MBS1753688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysatesLane 2: mouse heart tissue lysatesLane 3: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cyt 19/As3mt antigen affinity purified polyclonal antibody (Catalog # MBS1753688) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cyt 19/As3mt at approximately 44KD. The expected band size for Cyt 19/As3mt is at 42KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Cyt 19/As3mt antibody (MBS1753688).Overlay histogram showing A431 cells stained with MBS1753688 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyt 19/As3mt Antibody (MBS1753688,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Cyt 19/As3mt antibody (MBS1753688).Overlay histogram showing A431 cells stained with MBS1753688 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyt 19/As3mt Antibody (MBS1753688,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Related Product Information for anti-As3mt antibody
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. This gene is mapped to chromosome 10q24.
References
1. Hernandez, A., Xamena, N., Sekaran, C., Tokunaga, H., Sampayo-Reyes, A., Quinteros, D., Creus, A., Marcos, R. High arsenic metabolic efficiency in AS3MT-287Thr allele carriers. Pharmacogenet. Genomics 18: 349-355, 2008.
2. Lin, S., Shi, Q., Nix, F. B., Styblo, M., Beck, M. A., Herbin-Davis, K. M., Hall, L. L., Simeonsson, J. B., Thomas, D. J. A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol. J. Biol. Chem. 277: 10795-10803, 2002.
3. Wood, T. C., Salavagionne, O. E., Mukherjee, B., Wang, L., Klumpp, A. F., Thomae, B. A., Eckloff, B. W., Schaid, D. J., Wieben, E. D., Weinshilboum, R. M. Human arsenic methyltransferase (AS3MT) pharmacogenetics: Gene resequencing and functional genomics studies. J. Biol. Chem. 281: 7364-7373, 2006.
2. Lin, S., Shi, Q., Nix, F. B., Styblo, M., Beck, M. A., Herbin-Davis, K. M., Hall, L. L., Simeonsson, J. B., Thomas, D. J. A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol. J. Biol. Chem. 277: 10795-10803, 2002.
3. Wood, T. C., Salavagionne, O. E., Mukherjee, B., Wang, L., Klumpp, A. F., Thomae, B. A., Eckloff, B. W., Schaid, D. J., Wieben, E. D., Weinshilboum, R. M. Human arsenic methyltransferase (AS3MT) pharmacogenetics: Gene resequencing and functional genomics studies. J. Biol. Chem. 281: 7364-7373, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,794 Da
NCBI Official Full Name
arsenite methyltransferase
NCBI Official Synonym Full Names
arsenic (+3 oxidation state) methyltransferase
NCBI Official Symbol
As3mt
NCBI Official Synonym Symbols
Cyt19; 2310045H08Rik
NCBI Protein Information
arsenite methyltransferase; methyltransferase Cyt19; methylarsonite methyltransferase; S-adenosylmethionine:arsenic (III) methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase
UniProt Protein Name
Arsenite methyltransferase
Protein Family
UniProt Gene Name
As3mt
UniProt Synonym Gene Names
Cyt19
UniProt Entry Name
AS3MT_MOUSE
Similar Products
Product Notes
The As3mt as3mt (Catalog #AAA1753688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyt 19/As3mt Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyt 19/As3mt can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse| Direct ELISA: 0.1-0.5ug/ml|Mouse|. Researchers should empirically determine the suitability of the As3mt as3mt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyt 19/As3mt, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
