Rabbit ornithine aminotransferase/OAT Polyclonal Antibody | anti-OAT antibody

Anti-ornithine aminotransferase/OAT Antibody

Cat. Num. AAA1753401

• Gene Names: OAT; OKT; GACR; HOGA; OATASE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: ornithine aminotransferase/OAT; Polyclonal Antibody; Anti-ornithine aminotransferase/OAT Antibody; OAT; Ornithine aminotransferase; mitochondrial; EC 2. 6. 1. 13; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase [Cleaved into: Ornithine aminotransferase; hepatic form; renal form]; ornithine aminotransferase; anti-OAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for ornithine aminotransferase/OAT detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-OAT antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml|Human, Mouse, Rat|; IHC-P: 2-5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human OAT recombinant protein (Position: A214-F439).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-OAT antibody

Ornithine aminotransferase (OAT) is an enzyme which is encoded in human by the OAT gene located on chromosome 10. This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.

References

1. Akaki, Y., Hotta, Y., Mashima, Y., Murakami, A., Kennaway, N. G., Weleber, R. G., Inana, G. A deletion in the ornithine aminotransferase gene in gyrate atrophy. J. Biol. Chem. 267: 12950-12954, 1992.
2. Barrett, D. J., Bateman, J. B., Sparkes, R. S., Mohandas, T., Klisak, I., Inana, G. Chromosomal localization of human ornithine aminotransferase gene sequences to 10q26 and Xp11. 2. Invest. Ophthal. Vis. Sci. 28: 1037-1042, 1987.
3. Bisaillon, J. J., Radden, L. A., II, Szabo, E. T., Hughes, S. R., Feliciano, A. M., Nesta, A. V., Petrovic, B., Palanza, K. M., Lancinskas, D., Szmurlo, T. A., Artus, D. C., Kapper, M. A., Mulrooney, J. P., King, T. R. The retarded hair growth (rhg) mutation in mice is an allele of ornithine aminotransferase (Oat). Molec. Genet. Metab. Rep. 1: 378-390, 2014.

NCBI and Uniprot Product Information

• NCBI GI #: 4557809
• NCBI GeneID: 4942
• NCBI Accession #: NP_000265.1
• NCBI GenBank Nucleotide #: NM_000274.3
• UniProt Accession #: P04181; Q16068; Q16069; Q68CS0; Q6IAV9; Q9UD03; D3DRF0
• Molecular Weight: 48,535 Da
• NCBI Official Full Name: ornithine aminotransferase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: ornithine aminotransferase
• NCBI Official Symbol: OAT
• NCBI Official Synonym Symbols: OKT; GACR; HOGA; OATASE
• NCBI Protein Information: ornithine aminotransferase, mitochondrial; gyrate atrophy; ornithine delta-aminotransferase; ornithine-oxo-acid aminotransferase
• UniProt Protein Name: Ornithine aminotransferase, mitochondrial
• UniProt Gene Name: OAT
• UniProt Entry Name: OAT_HUMAN

NCBI Description

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]

Uniprot Description

OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding

Biological Process: visual perception; amino acid biosynthetic process

Disease: Gyrate Atrophy Of Choroid And Retina