Flow Cytometry (FC/FACS)
Rabbit anti-Mouse, Rat RANK/Tnfrsf11a Polyclonal Antibody | anti-Tnfrsf11a antibody
Anti-RANK/Tnfrsf11a Antibody
Gene Names
Tnfrsf11a; OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
Reactivity
Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
RANK/Tnfrsf11a; Polyclonal Antibody; Anti-RANK/Tnfrsf11a Antibody; Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; CD265; Tnfrsf11a; Rank; anti-Tnfrsf11a antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
625
Applicable Applications for anti-Tnfrsf11a antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
FC/FACS: 1-3ug/1x106 cells (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse RANK/Tnfrsf11a recombinant protein (Position: Q30-R203).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Western Blot (WB)
(Figure 1. Western blot analysis of Tnfrsf11a using anti-Tnfrsf11a antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Tnfrsf11a using anti-Tnfrsf11a antibody.)
Related Product Information for anti-Tnfrsf11a antibody
Description: Rabbit IgG polyclonal antibody for RANK/Tnfrsf11a detection. Tested with WB, FCM, Direct ELISA in Mouse; Rat.
Background: Receptor activator of nuclear factor kappa B (RANK), also known as TRANCE receptor or TNFRSF11A, is a member of the tumor. It is mapped to 1; 1 E2.1. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
Background: Receptor activator of nuclear factor kappa B (RANK), also known as TRANCE receptor or TNFRSF11A, is a member of the tumor. It is mapped to 1; 1 E2.1. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
References
1. Guerrini, M. M., Sobacchi, C., Cassani, B., Abinun, M., Kilic, S. S., Pangrazio, A., Moratto, D., Mazzolari, E., Clayton-Smith, J., Orchard, P., Coxon, F. P., Helfrich, M. H., and 9 others. Human osteoclast-poor osteopetrosis with hypogammaglobulinemia due to TNFRSF11A (RANK) mutations. Am. J. Hum. Genet. 83: 64-76, 2008.
2. Hughes, A. E., Ralston, S. H., Marken, J., Bell, C., MacPherson, H., Wallace, R. G. H., van Hul, W., Whyte, M. P., Nakatsuka, K., Hovy, L., Anderson, D. M. Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis. Nature Genet. 24: 45-48, 2000.
3. Johnson-Pais, T. L., Singer, F. R., Bone, H. G., McMurray, C. T., Hansen, M. F., Leach, R. J. Identification of a novel tandem duplication in exon 1 of the TNFRSF11A gene in two unrelated patients with familial expansile osteolysis. J. Bone Miner. Res. 18: 376-380, 2003.
2. Hughes, A. E., Ralston, S. H., Marken, J., Bell, C., MacPherson, H., Wallace, R. G. H., van Hul, W., Whyte, M. P., Nakatsuka, K., Hovy, L., Anderson, D. M. Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis. Nature Genet. 24: 45-48, 2000.
3. Johnson-Pais, T. L., Singer, F. R., Bone, H. G., McMurray, C. T., Hansen, M. F., Leach, R. J. Identification of a novel tandem duplication in exon 1 of the TNFRSF11A gene in two unrelated patients with familial expansile osteolysis. J. Bone Miner. Res. 18: 376-380, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 11A
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
NCBI Official Symbol
Tnfrsf11a
NCBI Official Synonym Symbols
OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
NCBI Protein Information
tumor necrosis factor receptor superfamily member 11A
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 11A
Protein Family
UniProt Gene Name
Tnfrsf11a
UniProt Synonym Gene Names
Rank; ODFR
UniProt Entry Name
TNR11_MOUSE
Uniprot Description
TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Protein type: Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; cytokine binding; metal ion binding
Biological Process: positive regulation of JNK activity; circadian thermoregulation; adaptive immune response; ossification; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; response to cytokine stimulus; programmed cell death; response to lipopolysaccharide; positive regulation of transcription factor activity; osteoclast differentiation; lymph node development; activation of NF-kappaB transcription factor
Research Articles on Tnfrsf11a
Similar Products
Product Notes
The Tnfrsf11a tnfrsf11a (Catalog #AAA1752963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RANK/Tnfrsf11a Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RANK/Tnfrsf11a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) FC/FACS: 1-3ug/1x106 cells (Mouse, Rat) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Tnfrsf11a tnfrsf11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANK/Tnfrsf11a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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