RANK/TNFRSF11A recombinant protein

Recombinant mouse RANK/TNFRSF11A protein

Cat. Num. AAA201240

• Gene Names: Tnfrsf11a; OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RANK/TNFRSF11A; Recombinant mouse RANK/TNFRSF11A protein; Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; activator of NFKB; FEO; OFE; OSTS; PDB2; RANK; RANKLOH18CR1; CD265; CD265 antigen; OPTB7; TRANCER; LOH18CR1; receptor activator of nuclear factor-kappa B; RANK/TNFRSF11A recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 31-214aa
• Sequence: VTPPCTQ ERHYEHLGRC CSRCEPGKYL SSKCTPTSDS VCLPCGPDEY LDTWNEEDKC LLHKVCDAGK ALVAVDPGNH TAPRRCACTA GYHWNSDCEC CRRNTECAPG FGAQHPLQLN KDTVCTPCLL GFFSDVFSST DKCKPWTNCT LLGKLEAHQG TTESDVVCSS SMTLRRPPKE AQAYLPS
• Sequence Length: 625

• Applicable Applications for RANK/TNFRSF11A recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Predicted Nterm: Ala48
• Tag Information: hIgG-His-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-Page

(SDS-Page)

Related Product Information for RANK/TNFRSF11A recombinant protein

RANK, as known as Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) is a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. It is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, immune cell function, lymph node development, thermal regulation, and mammary gland development. It is able to block TRANCE induced biological activity. Recombinant mouse RANK, fused to hlgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for RANK/TNFRSF11A recombinant protein

Immunology

References

Anderson, D.M. et al. (1997) Nature 390, 175-179.;; Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:395-400.;; Bharat B. Aggarwal. (2003) Nature 3, 745-756.;;

NCBI and Uniprot Product Information

• NCBI GI #: 110350009
• NCBI GeneID: 21934
• NCBI Accession #: NP_033425
• NCBI GenBank Nucleotide #: NP_033425.3
• UniProt Accession #: O35305
• Molecular Weight: 47.5 kDa (426aa)
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: Tnfrsf11a
• NCBI Official Synonym Symbols: OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• UniProt Gene Name: Tnfrsf11a
• UniProt Synonym Gene Names: Rank; ODFR
• UniProt Entry Name: TNR11_MOUSE

Uniprot Description

TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; cytokine binding; metal ion binding

Biological Process: positive regulation of JNK activity; circadian thermoregulation; adaptive immune response; ossification; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; response to cytokine stimulus; programmed cell death; response to lipopolysaccharide; positive regulation of transcription factor activity; osteoclast differentiation; lymph node development; activation of NF-kappaB transcription factor