Typical Testing Data/Standard Curve (for reference only)
Rhodamine ELISA Kit
Rhodamine Competitive ELISA Kit
Synonyms
Rhodamine; Rhodamine Competitive ELISA Kit; Rhodamine elisa kit
Preparation and Storage
Upon receipt, store Anti-Rhodamine Monoclonal Antibody (500X), Secondary Antibody-HRP Conjugate, Rhodamine Standard, 100X Conjugate Diluent, and Rhodamine Conjugate at -20 degree C. Store all the remaining components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Rhodamine elisa kit
Principle of the Assay: First, a rhodamine conjugate is coated on an ELISA plate. The unknown rhodamine samples or rhodamine standards are then added to the rhodamine conjugate preabsorbed ELISA plate. After a brief incubation, an anti-rhodamine antibody is added, followed by an HRP conjugated secondary antibody. The total content of rhodamine in unknown extracted samples is determined by comparison with a rhodamine standard curve.
Background/Introduction: Rhodamine encompasses a family of structurally related dyes that are derivatives of xanthene. Some important subtypes of the rhodamine family include Rhodamine 123, Rhodamine 6G, and Rhodamine B. They are often used to dye inks and paper. In addition, they are used as a tracer dye in order to find the rate or direction of flow and transport of water. Rhodamine is used as a collective marker in small mammals for studying movements. Rhodamine dyes are fluorescent and can therefore be easily and cheaply detected with a fluorometer. Rhodamines are even used in some lasers. Rhodamine dyes are often used in biological detection applications such as flow cytometry, fluorescence microscopy, ELISA, and fluorescence correlation spectroscopy. The subtype Rhodamine 123 is used in biochemistry to inhibit the function of mitochondria. Rhodamine 123 inhibits transport processes such as the electron transport chain and slows down inner respiration by binding to the mitochondrial membranes. Rhodamine 123 is also a substrate of P-glycoprotein (usually overexpressed in cancer cells) as well as of multidrug resistance-associated protein (MRP1). There are many rhodamine derivatives used for imaging purposes, such as tetramethylrhodamine (TMR) and its isothiocyanate derivative (TRITC), carboxytetramethylrhodamine (TAMRA), as well as sulforhodamine 101 (and its sulfonyl chloride form Texas Red), and Rhodamine Red. Cell Biolabs' Rhodamine Competitive ELISA Kit provides a convenient method for the detection of total rhodamine in extracts from cells, tissue, serum, plasma, or foods. The total content of rhodamine in unknown samples is determined by comparison with a rhodamine standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 1 uM rhodamine.
Background/Introduction: Rhodamine encompasses a family of structurally related dyes that are derivatives of xanthene. Some important subtypes of the rhodamine family include Rhodamine 123, Rhodamine 6G, and Rhodamine B. They are often used to dye inks and paper. In addition, they are used as a tracer dye in order to find the rate or direction of flow and transport of water. Rhodamine is used as a collective marker in small mammals for studying movements. Rhodamine dyes are fluorescent and can therefore be easily and cheaply detected with a fluorometer. Rhodamines are even used in some lasers. Rhodamine dyes are often used in biological detection applications such as flow cytometry, fluorescence microscopy, ELISA, and fluorescence correlation spectroscopy. The subtype Rhodamine 123 is used in biochemistry to inhibit the function of mitochondria. Rhodamine 123 inhibits transport processes such as the electron transport chain and slows down inner respiration by binding to the mitochondrial membranes. Rhodamine 123 is also a substrate of P-glycoprotein (usually overexpressed in cancer cells) as well as of multidrug resistance-associated protein (MRP1). There are many rhodamine derivatives used for imaging purposes, such as tetramethylrhodamine (TMR) and its isothiocyanate derivative (TRITC), carboxytetramethylrhodamine (TAMRA), as well as sulforhodamine 101 (and its sulfonyl chloride form Texas Red), and Rhodamine Red. Cell Biolabs' Rhodamine Competitive ELISA Kit provides a convenient method for the detection of total rhodamine in extracts from cells, tissue, serum, plasma, or foods. The total content of rhodamine in unknown samples is determined by comparison with a rhodamine standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 1 uM rhodamine.
Product Categories/Family for Rhodamine elisa kit
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Product Notes
The Rhodamine (Catalog #AAA169593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Rhodamine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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