Testing Data
(Figure 1. Recombinant mCherry on SDS- PAGE . Lane A (unheated and without DTT) and Lane B (boiled containing DTT). Note: SDS denaturation and boiling is known to produce a 22kDa cleavage product (due to hydrolysis of the main chain acylimine linkage ).)
RFP Active Protein
Recombinant RFP
Synonyms
RFP; Recombinant RFP; RFP active protein
Form/Format
100 ug/vial at 1.0 mg/mL in 1X PBS
Purity and Activity
Expressed and purified from E.Coli as a fusion protein, greater than 95% by SDS -PAGE (Figure 1). Cell Biolabs’ mCherry has an approximate MW of 29 kDa, containing a 6xHis-tag at the C-terminus, and an excitation/emission spectra of 587/610 nm. Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) (Figure 2)
Preparation and Storage
Store at -80°C; avoid freeze/thaw.
Testing Data
(Figure 1. Recombinant mCherry on SDS- PAGE . Lane A (unheated and without DTT) and Lane B (boiled containing DTT). Note: SDS denaturation and boiling is known to produce a 22kDa cleavage product (due to hydrolysis of the main chain acylimine linkage ).)
Testing Data
(Figure 1. Recombinant mCherry on SDS- PAGE . Lane A (unheated and without DTT) and Lane B (boiled containing DTT). Note: SDS denaturation and boiling is known to produce a 22kDa cleavage product (due to hydrolysis of the main chain acylimine linkage ).)
Testing Data #2
(Figure 2 . mCherry Fluorometric Activity . Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 584/612 nm filter set. One should use the data below for reference only. This data should not be used to interpret actual results)
Testing Data #2
(Figure 2 . mCherry Fluorometric Activity . Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 584/612 nm filter set. One should use the data below for reference only. This data should not be used to interpret actual results)
Related Product Information for RFP active protein
• Includes 6xHis-tag at C-terminus
• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBS
Red fluorescent protein (DsRed) is a spontaneously fluorescent protein isolated from the Indo-Pacific sea coral, Discosoma striata. It absorbs and emits orange-red light and is well suited for multi-color tagging used in FRET. Since the molecular cloning of RFP cDNA and demonstration of RFP as a functional transgene, RFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. RFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the RFP molecule, many RFP variants, such as TagRFP, TurboRFP, DsRed, tdTomato, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, and mStrawberry, have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rRFP requires no additional substrates or cofactors, rRFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.
• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBS
Red fluorescent protein (DsRed) is a spontaneously fluorescent protein isolated from the Indo-Pacific sea coral, Discosoma striata. It absorbs and emits orange-red light and is well suited for multi-color tagging used in FRET. Since the molecular cloning of RFP cDNA and demonstration of RFP as a functional transgene, RFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. RFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the RFP molecule, many RFP variants, such as TagRFP, TurboRFP, DsRed, tdTomato, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, and mStrawberry, have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rRFP requires no additional substrates or cofactors, rRFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.
Product Categories/Family for RFP active protein
References
1. Shaner, N.C. et al, J. Cell Sci. 120: 4247-4260, 2007.
2. Matz, M.V. et al, Nat. Biotechnol. 17: 969-973, 1999.
3. Campbell, R.E. et al, Proc. Natl. Acad. Sci. USA 99: 7877-7882, 2002.
4. Shaner, N.C. et al, Nat. Biotechnol. 22: 1567-1572, 2004.
2. Matz, M.V. et al, Nat. Biotechnol. 17: 969-973, 1999.
3. Campbell, R.E. et al, Proc. Natl. Acad. Sci. USA 99: 7877-7882, 2002.
4. Shaner, N.C. et al, Nat. Biotechnol. 22: 1567-1572, 2004.
Similar Products
Product Notes
The RFP (Catalog #AAA168749) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RFP, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
