Typical Standard Curve/Testing Data
Human Free PSA ELISA Kit | f-PSA elisa kit
Human Free PSA (f-PSA) ELISA Kit
Reactivity
Human
Synonyms
Free PSA; Human Free PSA (f-PSA) ELISA Kit; f-PSA elisa kit
Reactivity
Human
Preparation and Storage
4 degree C
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for f-PSA elisa kit
Principle of the Assay: An anti-f-PSA coating antibody is adsorbed onto a microtiter plate. Free PSA protein present in the sample or standard binds to the antibodies adsorbed on the plate; a biotinylated anti-f-PSA antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a streptavidin-enzyme conjugate is added and binds to the biotinylated anti-f-PSA antibody. Unbound streptavidin-enzyme conjugate is removed during a wash step, and substrate solution is added to the wells. A colored product is formed in proportion to the amount of f-PSA present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from a f-PSA calibrator and sample concentration is then determined.
Background/Introduction: Prostate-Specific Antigen (PSA) is a 33 kDa serine proteinase secreted by epithelial cells of the prostate gland. PSA exists in serum in 3 main forms: unbound free PSA (f-PSA), alpha1-antichymotrypsin bound PSA, and alpha2-macroglobulin bound PSA. Blood PSA levels are typically low in men with healthy prostates, but are often elevated in individuals with prostate disorders (i.e. cancer, prostatitis, benign prostate hyperplasia). Measuring the ratio of free to total PSA (t-PSA) is an effective tool in prostate cancer screening and identification. Free PSA ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the human f-PSA protein. The kit has a detection sensitivity limit of 15 pg/mL and contains a HYBRITECH f-PSA Calibration Standard. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and f-PSA samples.
Background/Introduction: Prostate-Specific Antigen (PSA) is a 33 kDa serine proteinase secreted by epithelial cells of the prostate gland. PSA exists in serum in 3 main forms: unbound free PSA (f-PSA), alpha1-antichymotrypsin bound PSA, and alpha2-macroglobulin bound PSA. Blood PSA levels are typically low in men with healthy prostates, but are often elevated in individuals with prostate disorders (i.e. cancer, prostatitis, benign prostate hyperplasia). Measuring the ratio of free to total PSA (t-PSA) is an effective tool in prostate cancer screening and identification. Free PSA ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the human f-PSA protein. The kit has a detection sensitivity limit of 15 pg/mL and contains a HYBRITECH f-PSA Calibration Standard. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and f-PSA samples.
Product Categories/Family for f-PSA elisa kit
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Product Notes
The Human f-PSA (Catalog #AAA169308) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA169308 ELISA Kit recognizes Human f-PSA. It is sometimes possible for the material contained within the vial of "Free PSA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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