Typical Testing Data/Standard Curve (for reference only)
Fluorescein ELISA Kit
Fluorescein Competitive ELISA Kit
Synonyms
Fluorescein; Fluorescein Competitive ELISA Kit; Fluorescein elisa kit
Preparation and Storage
Upon receipt, store Anti-Fluorescein Antibody (500X), Secondary Antibody-HRP Conjugate, Fluorescein Standard, 100X Conjugate Diluent, and Fluorescein Conjugate at -20 degree C. Store all the remaining components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Fluorescein elisa kit
Principle of the Assay: First, a fluorescein conjugate is coated on an ELISA plate. The unknown fluorescein samples or fluorescein standards are then added to the fluorescein conjugate preabsorbed ELISA plate. After a brief incubation, an anti-fluorescein antibody is added, followed by an HRP conjugated secondary antibody. The total content of fluorescein (or FITC) in unknown extracted samples is determined by comparison with a fluorescein standard curve.
Background/Introduction: Fluorescein is a dye often used as a fluorescent tracer. It has a 494 nm absorption maximum and an emission maximum of 512 nm in water. Fluorescein isothiocyanate (FITC) and 6-FAM phosphoramidite (the latter used in oligonucleotide synthesis) are the two major derivatives of fluorescein. FITC is commonly used to attach to biologically active molecules such as antibodies. These conjugates are used to label and track cells in fluorescence microscopy applications such as flow cytometry or immunofluorescent staining. Fluorescein can also be conjugated to nucleoside triphosphates and incorporated into a DNA probe enzymatically for in situ hybridization experiments. Sodium fluorescein is also employed to identify aberrations in the field of ophthalmology and optometry, where topical fluorescein can elucidate corneal abrasions, corneal ulcers and herpetic corneal infections. Fluorescein can also be used in rigid gas permeable contact lens fittings to evaluate the tear layer under the lens. Cell Biolabs' Fluorescein Competitive ELISA Kit provides a convenient method for the detection of total fluorescein in extracts from cells, tissue, serum, plasma, or foods. The total content of fluorescein in unknown samples is determined by comparison with a fluorescein standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 20 pM fluorescein.
Background/Introduction: Fluorescein is a dye often used as a fluorescent tracer. It has a 494 nm absorption maximum and an emission maximum of 512 nm in water. Fluorescein isothiocyanate (FITC) and 6-FAM phosphoramidite (the latter used in oligonucleotide synthesis) are the two major derivatives of fluorescein. FITC is commonly used to attach to biologically active molecules such as antibodies. These conjugates are used to label and track cells in fluorescence microscopy applications such as flow cytometry or immunofluorescent staining. Fluorescein can also be conjugated to nucleoside triphosphates and incorporated into a DNA probe enzymatically for in situ hybridization experiments. Sodium fluorescein is also employed to identify aberrations in the field of ophthalmology and optometry, where topical fluorescein can elucidate corneal abrasions, corneal ulcers and herpetic corneal infections. Fluorescein can also be used in rigid gas permeable contact lens fittings to evaluate the tear layer under the lens. Cell Biolabs' Fluorescein Competitive ELISA Kit provides a convenient method for the detection of total fluorescein in extracts from cells, tissue, serum, plasma, or foods. The total content of fluorescein in unknown samples is determined by comparison with a fluorescein standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 20 pM fluorescein.
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Product Notes
The Fluorescein (Catalog #AAA169592) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fluorescein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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