Typical Testing Data/Standard Curve (for reference only)
Acetylcholine Assay Kit
Acetylcholine Assay Kit (Colorimetric)
Synonyms
Acetylcholine; Acetylcholine Assay Kit (Colorimetric); Acetylcholine assay kit
Preparation and Storage
Upon receipt, store the Assay Buffer (10X) at 4 degree C. Store the remaining components at -20 degree C. The Colorimetric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Acetylcholine assay kit
Principle of the Assay: Acetylcholine Assay Kit measures the acetylcholine present within serum, plasma, or tissue samples. The assay is based on the enzyme driven reaction that will detect acetylcholine via acetylcholinesterase enzyme and choline oxidase. First, acetylcholinesterase hydrolyzes acetylcholine into choline and acetic acid. Choline is then oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorescence probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of acetylcholine standard within the 96-well microtiter plate format. Samples and standards are incubated for 45 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Acetylcholine is a polyatomic cation neurotransmitter that is produced in acetylcholinergic neurons. It is one of many neurotransmitters within the autonomic nervous system and the only neurotransmitter in the motor function of the somatic nervous system. Acetylcholine works within the peripheral and central nervous systems within many organisms, including humans. It is manufactured via choline acetyltransferase from acetyl-CoA and choline. Choline is an amine that is an essential nutrient that is a key precursor to many phospholipids. Acetylcholine works in the peripheral nervous system by activating skeletal muscles as well as smooth muscle and cardiac muscle function. Within the central nervous system, acetylcholine acts as a neuromodulator for the cholinergic system, which causes excitatory actions. Here the neurotransmitter is involved with plasticity, excitability, arousal, and reward. Acetylcholine's half-life and activity are very short because it is broken down by acetylcholinesterase. There are two main acetylcholine receptors: nicotinic and muscarinic. Acetylcholine disorders can have a profound impact on neurological function. A shortage of acetylcholine, such as the autoimmune disorder Myasthenia gravis, leads to muscle fatigue and weakness due to antibodies blocking acetylcholine receptors. Acetylcholine has also been implicated in many disease states including diabetic vasculopathy, hypertension, and Alzheimer's disease. Acetylcholine Assay Kit is a simple fluorometric assay that measures the amount of acetylcholine present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample acetylcholine concentrations are determined by comparison with a known acetylcholine standard. The kit's detection sensitivity limit is approximately 0.05 uM acetylcholine.
Background/Introduction: Acetylcholine is a polyatomic cation neurotransmitter that is produced in acetylcholinergic neurons. It is one of many neurotransmitters within the autonomic nervous system and the only neurotransmitter in the motor function of the somatic nervous system. Acetylcholine works within the peripheral and central nervous systems within many organisms, including humans. It is manufactured via choline acetyltransferase from acetyl-CoA and choline. Choline is an amine that is an essential nutrient that is a key precursor to many phospholipids. Acetylcholine works in the peripheral nervous system by activating skeletal muscles as well as smooth muscle and cardiac muscle function. Within the central nervous system, acetylcholine acts as a neuromodulator for the cholinergic system, which causes excitatory actions. Here the neurotransmitter is involved with plasticity, excitability, arousal, and reward. Acetylcholine's half-life and activity are very short because it is broken down by acetylcholinesterase. There are two main acetylcholine receptors: nicotinic and muscarinic. Acetylcholine disorders can have a profound impact on neurological function. A shortage of acetylcholine, such as the autoimmune disorder Myasthenia gravis, leads to muscle fatigue and weakness due to antibodies blocking acetylcholine receptors. Acetylcholine has also been implicated in many disease states including diabetic vasculopathy, hypertension, and Alzheimer's disease. Acetylcholine Assay Kit is a simple fluorometric assay that measures the amount of acetylcholine present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample acetylcholine concentrations are determined by comparison with a known acetylcholine standard. The kit's detection sensitivity limit is approximately 0.05 uM acetylcholine.
Product Categories/Family for Acetylcholine assay kit
References
1. Gilberstadt, M.L., et al. (1984) Anal. Biochem. 138: 78-85.
2. Holm, P.I., et al. (2003) Clin. Chem. 49: 286-294.
3. Kovarik, Z., et al. (2003) Biochem. J. 373: 33-40.
4. Magnottl, R.A., et al. (1987) Clin. Chem. 33/10: 1731-1735.
5. Vizi, E.S., et al. (1985) J. Pharmacol. Methods 13: 201-211.
2. Holm, P.I., et al. (2003) Clin. Chem. 49: 286-294.
3. Kovarik, Z., et al. (2003) Biochem. J. 373: 33-40.
4. Magnottl, R.A., et al. (1987) Clin. Chem. 33/10: 1731-1735.
5. Vizi, E.S., et al. (1985) J. Pharmacol. Methods 13: 201-211.
Similar Products
Product Notes
The Acetylcholine (Catalog #AAA169077) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Acetylcholine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
