Testing Data
pCMV-VSV-G Retroviral Vector
pCMV-VSV-G Retroviral Vector
Synonyms
pCMV-VSV-G; pCMV-VSV-G Retroviral Vector; pCMV-VSV-G Envelope Vector; pCMV-VSV-G retroviral vector
Quantity
10 ug at 0.25 ug/uL in TE
Safety Considerations
Remember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.
Preparation and Storage
Store at -20°C
Testing Data
Testing Data
Related Product Information for pCMV-VSV-G retroviral vector
• Use this vector along with a packaging cell line and a cloning / expression vector for retroviral production.
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus (VSV-G) under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus (MMLV)-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipid components of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria (Figure 1).
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus (VSV-G) under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus (MMLV)-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipid components of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria (Figure 1).
Product Categories/Family for pCMV-VSV-G retroviral vector
References
1. Emi, N., et al., (1991) J. Virol. 65, 1202-1207.
1. Abram, M. E. et al. (2015). A cell-based strategy to assess intrinsic inhibition efficiencies of HIV-1 reverse transcriptase inhibitors. Antimicrob Agents Chemother. 59:838-848.
2. Amagai, Y. et al. (2013). Stem cell factor contributes to tumorigenesis of mast cells via an autocrine/paracrine mechanism. J. Leukoc. Biol. 93:245-250.
3. Okamoto, K. et al. (2012). Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. J.Gen. Virol. 93:761-770.
4. Oida, T. et al. (2010). Overexpression of TGF-beta1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-beta. J. Immunol. 185:3529-3535.
1. Abram, M. E. et al. (2015). A cell-based strategy to assess intrinsic inhibition efficiencies of HIV-1 reverse transcriptase inhibitors. Antimicrob Agents Chemother. 59:838-848.
2. Amagai, Y. et al. (2013). Stem cell factor contributes to tumorigenesis of mast cells via an autocrine/paracrine mechanism. J. Leukoc. Biol. 93:245-250.
3. Okamoto, K. et al. (2012). Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. J.Gen. Virol. 93:761-770.
4. Oida, T. et al. (2010). Overexpression of TGF-beta1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-beta. J. Immunol. 185:3529-3535.
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Product Notes
The pCMV-VSV-G (Catalog #AAA168028) is a Retroviral Vector and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "pCMV-VSV-G, Retroviral Vector" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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