Testing Data
pBABE-Hygro Retroviral Vector
pBABE-Hygro Retroviral Vector
Synonyms
pBABE-Hygro; pBABE-Hygro Retroviral Vector; pBABE-Hygro retroviral vector
Quantity
10 ug at 0.5 ug/uL in TE
Safety Considerations
Remember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.
Preparation and Storage
-20 degree C
Testing Data
Testing Data
Related Product Information for pBABE-Hygro retroviral vector
• 10 ug of purified plasmid
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. MyBioSource's retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelope protein, which determines the viral infectivity range. Transfection into a package cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo.
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and hygromycin-resistance gene for the growth of infected mammalian cells to select stable cell lines (Figure 1).
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. MyBioSource's retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelope protein, which determines the viral infectivity range. Transfection into a package cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo.
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and hygromycin-resistance gene for the growth of infected mammalian cells to select stable cell lines (Figure 1).
Product Categories/Family for pBABE-Hygro retroviral vector
References
1. Morgenstern, J. P. and H Land. (1990) Nuc. Acid Res. 18, 3587-3596.
2. Coffin, J. M. and H. E. Varmus, Retroviruses, Cold Spring Harbor Press, NY.
3. Schuck S, Manninen A, Honsho M, Fullekrug J and Simons K. (2004) Proc Natl Acad Sci U S A. 101, 4912-4917.
1. Duran, P.P. et al. (2012) UNG Shapes the Specificity of AID-Induced Somatic Hypermutation. J. Exp. Med. 209: 1379-1389.
2. Ding, J. et al. (2010). Bcl-2 and Bax Interact via the BH1-
3 Groove-BH3 Motif Interface and a Novel Interface Involving the BH4 Motif. J. Biol. Chem. 285:28749-28763.
2. Coffin, J. M. and H. E. Varmus, Retroviruses, Cold Spring Harbor Press, NY.
3. Schuck S, Manninen A, Honsho M, Fullekrug J and Simons K. (2004) Proc Natl Acad Sci U S A. 101, 4912-4917.
1. Duran, P.P. et al. (2012) UNG Shapes the Specificity of AID-Induced Somatic Hypermutation. J. Exp. Med. 209: 1379-1389.
2. Ding, J. et al. (2010). Bcl-2 and Bax Interact via the BH1-
3 Groove-BH3 Motif Interface and a Novel Interface Involving the BH4 Motif. J. Biol. Chem. 285:28749-28763.
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Product Notes
The pBABE-Hygro (Catalog #AAA168576) is a Retroviral Vector and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "pBABE-Hygro, Retroviral Vector" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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