Testing Data
pBABE-Puro Retroviral Vector
pBABE-Puro Retroviral Vector
Synonyms
pBABE-Puro; pBABE-Puro Retroviral Vector; pBABE-Puro retroviral vector
Quantity
10 ug at 0.5 ug/uL in TE
Safety Considerations
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and puromycin-resistance gene for the grwoth of infected mammalian cells to select stable cell lines (Figure 1).
Preparation and Storage
-20 degree C
Testing Data
Testing Data
Related Product Information for pBABE-Puro retroviral vector
• 10 ug of purified plasmid
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. MyBioSource's retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelope protein, which determines the viral infectivity range. Transfection into a package cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo.
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and puromycin-resistance gene for the grwoth of infected mammalian cells to select stable cell lines (Figure 1).
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. MyBioSource's retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelope protein, which determines the viral infectivity range. Transfection into a package cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo.
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and puromycin-resistance gene for the grwoth of infected mammalian cells to select stable cell lines (Figure 1).
Product Categories/Family for pBABE-Puro retroviral vector
References
1. Morgenstern, J. P. and H Land. (1990) Nuc. Acid Res. 18, 3587-3596.
2. Coffin, J. M. and H. E. Varmus, Retroviruses, Cold Spring Harbor Press, NY.
3. Schuck S, Manninen A, Honsho M, Fullekrug J and Simons K. (2004) Proc Natl Acad Sci U S A. 101, 4912-4917.
1. Tomasovic, A. et al. (2015). Sestrin 2 regulates PDGF receptor beta (PDGFRbeta) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. doi:10.1074/jbc.M114.632133.
2. Diep, C. M. et al. (2015). Retroviral expression of human cystatin genes in HeLa cells. Methods Mol Bio. 1249:121-131.
3. Ramos-Montoya, A. et al. (2014). HES6 drives a critical ar transcriptional programme to induce castration-resistant prostate cancer through activation of an E2F1-mediated cell cycle network. EMBO Mol Med. 6:651-661.
4. Pagnon, J. et al. (2012). Identification and functional characterization of Protein Kinase A phosphorylation sites in the major lipolytic protein, adipose triglyceride lipase. Endocrinology. 153:4278-4289.
5. Zheng,Yu et al. (2012). Protein-Tyrosine Kinase 6 promotes peripheral adhesion complex formation and cell migration by phosphorylating p130 CRK-associated substrate. J. Biol. Chem. 287:148-158.
6. Jeong, H. et al. (2010). TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. FASEB J. 10.1096/fj.09-151324.
2. Coffin, J. M. and H. E. Varmus, Retroviruses, Cold Spring Harbor Press, NY.
3. Schuck S, Manninen A, Honsho M, Fullekrug J and Simons K. (2004) Proc Natl Acad Sci U S A. 101, 4912-4917.
1. Tomasovic, A. et al. (2015). Sestrin 2 regulates PDGF receptor beta (PDGFRbeta) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. doi:10.1074/jbc.M114.632133.
2. Diep, C. M. et al. (2015). Retroviral expression of human cystatin genes in HeLa cells. Methods Mol Bio. 1249:121-131.
3. Ramos-Montoya, A. et al. (2014). HES6 drives a critical ar transcriptional programme to induce castration-resistant prostate cancer through activation of an E2F1-mediated cell cycle network. EMBO Mol Med. 6:651-661.
4. Pagnon, J. et al. (2012). Identification and functional characterization of Protein Kinase A phosphorylation sites in the major lipolytic protein, adipose triglyceride lipase. Endocrinology. 153:4278-4289.
5. Zheng,Yu et al. (2012). Protein-Tyrosine Kinase 6 promotes peripheral adhesion complex formation and cell migration by phosphorylating p130 CRK-associated substrate. J. Biol. Chem. 287:148-158.
6. Jeong, H. et al. (2010). TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. FASEB J. 10.1096/fj.09-151324.
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Product Notes
The pBABE-Puro (Catalog #AAA169150) is a Retroviral Vector and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "pBABE-Puro, Retroviral Vector" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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