Human Protein max ELISA Kit | MAX elisa kit

Human Protein max ELISA Kit

Cat. Num. AAA765982

• Gene Names: Max; bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; AA960152; AI875693
• Reactivity: Human
• Synonyms: Protein max; Human Protein max ELISA Kit; MAX/BHLHD4 (Protein max)/bHLHd5/bHLHd6/bHLHd7/bHLHd8/Class D basic helix-loop-helix protein 4/helix-loop-helix zipper protein/MAX protein/MYC associated factor X/Myc-associated factor X/orf1; MAX elisa kit

• Reactivity: Human
• Sequence Length: 151

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAX elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MAX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MAX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MAX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MAX can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 226051848
• NCBI GeneID: 17187
• NCBI Accession #: NP_001139648.1
• NCBI GenBank Nucleotide #: NM_001146176.1
• Molecular Weight: 18,197 Da
• NCBI Official Full Name: protein max isoform 2
• NCBI Official Synonym Full Names: Max protein
• NCBI Official Symbol: Max
• NCBI Official Synonym Symbols: bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; AA960152; AI875693
• NCBI Protein Information: protein max
• UniProt Protein Name: Protein max
• Protein Family: Protein max
• UniProt Gene Name: Max
• UniProt Synonym Gene Names: Myn
• UniProt Entry Name: MAX_MOUSE

Uniprot Description

MAX: a transcription factor. Forms a DNA- binding protein complex with MYC or MAD. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. CPG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC/MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3-K9 histone methyltransferase activity. Three alternatively spliced isoforms have been reported.

Protein type: Transcription factor; DNA-binding

Cellular Component: cell projection; cytoplasm; dendrite; nucleoplasm; nucleus; PML body

Molecular Function: DNA binding; protein binding; protein complex binding; protein dimerization activity; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: protein complex assembly; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent