RFP Lentivirus Control Lentiviral Vector

RFP Lentivirus Control

Cat. Num. AAA168425

• Synonyms: RFP Lentivirus Control; RFP Lentivirus Control lentiviral vector

• Concentration: 200 uL, >1 x 10^6 TU/mL in DMEM, 10% FBS, Pen-Strep (varies by lot)

• Quality Control: This vial contains 200 uL of control RFP supernatant with p24 level of 1 ug/mL (particle titer) as determined by a p24 ELISA kit and at least 1.0 × 10^6 TU/mL (infectious titer) as determined by red fluorescence of infected 293 cells (Figure 2).
• Safety Considerations: Remember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Precautions must be taken to avoid direct contact with viral supernatants.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: -80 degree C, Avoid repeat freeze/thaw cycles

Testing Data

Related Product Information for RFP Lentivirus Control lentiviral vector

• Provided as 200 uL of lentiviral supernatant at >1 x 10^6 TU/mL

Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo.

The provided RFP lentivirus control is a VSVG-pseudotyped pantropic virus capable of infecting both dividing and non-dividing cells. The virus contains RFP and Puromycin resistant genes (Figure 1), it provides a useful control for transduction. This control virus can also be used to generate RFP stable cell lines, and stable clones can be selected by either red fluorescence sorting or Puromycin resistance in 1 to 2 ug/mL Puromycin-containing medium.

Product Categories/Family for RFP Lentivirus Control lentiviral vector

Cell Signaling and Protein Biology; Reporter Assays; Cells and Reagents; Reporter Recombinant Viruses

References

1. Naldini, L., U. Blomer, P. Gallay, D. Ory, R. Mulligan, F. H. Gage, I. M. Verma, and D. Trono (1996) Science 272, 263-267.
2. Verma, I. M., and N. Somia (1997) Nature 389, 239-242
3. Kahl C. A., Marsh J., Fyffe J., Sanders D. A., and K. Cornetta (2004) J Virol. 78:1421-30.
4. White S. M., Renda M., Nam N. Y., Klimatcheva E., Zhu Y., Fisk J., Halterman M., Rimel B. J., Federoff H., Pandya S., Rosenblatt J. D., and V. Planelles (1999) J Virol. 73:2832-40.
5. Kafri T., van Praag H., Ouyang L., Gage F. H., and I. M. Verma (1999) J Virol. 73:576-84.