Western Blot (WB)
(Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit SHROOM1 Polyclonal Antibody | anti-SHROOM1 antibody
SHROOM1 Antibody
Gene Names
SHROOM1; APXL2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
SHROOM1 Antibody is affinity chromatography purified via peptide column.
Synonyms
SHROOM1; Polyclonal Antibody; SHROOM1 Antibody; APXL2; KIAA1960; Protein Shroom1; Apical protein 2; shroom family member 1; anti-SHROOM1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members.
Purity/Purification
SHROOM1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
852
Applicable Applications for anti-SHROOM1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 mug/mL.
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal SHROOM1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SHROOM1.
Buffer
SHROOM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SHROOM1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-SHROOM1 antibody
SHROOM1 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94 kDa
NCBI Official Full Name
protein Shroom1 isoform 1
NCBI Official Synonym Full Names
shroom family member 1
NCBI Official Symbol
SHROOM1
NCBI Official Synonym Symbols
APXL2
NCBI Protein Information
protein Shroom1; apical protein 2
UniProt Protein Name
Protein Shroom1
Protein Family
UniProt Gene Name
SHROOM1
UniProt Synonym Gene Names
APXL2; KIAA1960
UniProt Entry Name
SHRM1_HUMAN
NCBI Description
SHROOM family members play diverse roles in the development of the nervous system and other tissues (Hagens et al., 2006 [PubMed 16615870]).[supplied by OMIM, Mar 2008]
Uniprot Description
SHROOM1: May be involved in the assembly of microtubule arrays during cell elongation. Belongs to the shroom family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: microtubule; myosin II complex; cytoplasm
Molecular Function: actin filament binding
Biological Process: actin filament bundle formation; cell morphogenesis
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Product Notes
The SHROOM1 shroom1 (Catalog #AAA153672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHROOM1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHROOM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the SHROOM1 shroom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHROOM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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