Western Blot (WB)
(Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.)
Rabbit anti-Human SHROOM3 Polyclonal Antibody | anti-SHROOM3 antibody
SHROOM3 Antibody
Gene Names
SHROOM3; SHRM; APXL3; ShrmL; MSTP013
Reactivity
Human
Applications
ELISA, Western Blot
Purity
SHROOM3 Antibody is affinity chromatography purified via peptide column.
Synonyms
SHROOM3; Polyclonal Antibody; SHROOM3 Antibody; SHRM; APXL3; ShrmL; MSTP013; KIAA1481; SHRML; Protein Shroom3; Shroom-related protein; hShrmL; shroom family member 3; anti-SHROOM3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
SHROOM3 antibody is human specific. At least four isoforms of SHROOM3 are known to exist; this antibody will detect all four isoforms. SHROOM3 antibody is predicted to not cross-react with other SHROOM family members.
Purity/Purification
SHROOM3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1996
Applicable Applications for anti-SHROOM3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
SHROOM3 antibody can be used for detection of SHROOM3 by Western blot at 1 - 2 mug/mL.
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal SHROOM3 antibody was raised against an 18 amino acid peptide near the center of human SHROOM3.
Buffer
SHROOM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SHROOM3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.)
Related Product Information for anti-SHROOM3 antibody
SHROOM3 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM3 is an actin-binding protein is a regulator of the microtubule cytoskeleton during epithelial morphogenesis and is sufficient and necessary to induce a redistribution of the microtubule regulator gamma-tubulin. It is necessary for both apiscobasal cell elongation and apical constriction in the neural epithelium. SHROOM3 has also been shown to be essential for cell shape changes and morphogenesis in the developing vertebrate gut, with its transcription relying on the Pitx1 transcription factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
220 kDa
NCBI Official Full Name
protein Shroom3
NCBI Official Synonym Full Names
shroom family member 3
NCBI Official Symbol
SHROOM3
NCBI Official Synonym Symbols
SHRM; APXL3; ShrmL; MSTP013
NCBI Protein Information
protein Shroom3; shroom-related protein; F-actin-binding protein
UniProt Protein Name
Protein Shroom3
Protein Family
UniProt Gene Name
SHROOM3
UniProt Synonym Gene Names
KIAA1481; SHRML; hShrmL
UniProt Entry Name
SHRM3_HUMAN
NCBI Description
This gene encodes a PDZ-domain-containing protein that belongs to a family of Shroom-related proteins. This protein may be involved in regulating cell shape in certain tissues. A similar protein in mice is required for proper neurulation. [provided by RefSeq, Jan 2011]
Uniprot Description
SHRM: a filamentous-actin binding protein. Required for normal neural tube closure and cytoskeletal polarity in developing neuroepithelial cells.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: apical junction complex; microtubule; cytoskeleton; adherens junction; apical plasma membrane; cytoplasm
Molecular Function: actin filament binding
Biological Process: regulation of cell shape; apical protein localization; neural tube closure; cell morphogenesis; cellular pigment accumulation; columnar/cuboidal epithelial cell development; actin cytoskeleton organization and biogenesis; pattern specification process
Research Articles on SHROOM3
Similar Products
Product Notes
The SHROOM3 shroom3 (Catalog #AAA153584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHROOM3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHROOM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). SHROOM3 antibody can be used for detection of SHROOM3 by Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the SHROOM3 shroom3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHROOM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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