Rabbit anti-Human, Rat TYW1 Polyclonal Antibody | anti-TYW1 antibody

TYW1 Antibody

Cat. Num. AAA151378

• Gene Names: TYW1; TYW1A; RSAFD1; YPL207W
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: TYW1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TYW1; Polyclonal Antibody; TYW1 Antibody; TYW1A; RSAFD1; YPL207W; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; tRNA-yW synthesizing protein 1 homolog (S. cerevisiae); anti-TYW1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms.
• Purity/Purification: TYW1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 732

• Applicable Applications for anti-TYW1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: TYW1 antibody can be used for detection of TYW1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1.
• Buffer: TYW1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TYW1 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 μg/mL.)

Related Product Information for anti-TYW1 antibody

TYW1 Antibody: TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions

NCBI and Uniprot Product Information

• NCBI GI #: 50726981
• NCBI GeneID: 55253
• NCBI Accession #: NP_060734
• NCBI GenBank Nucleotide #: NM_018264.3
• UniProt Accession #: Q9NV66; Q6PJG8; Q75MG8; Q75MN3; Q86V12; Q8IVS7; Q9H9C4
• Molecular Weight: 43,700 Da
• NCBI Official Full Name: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
• NCBI Official Synonym Full Names: tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
• NCBI Official Symbol: TYW1
• NCBI Official Synonym Symbols: TYW1A; RSAFD1; YPL207W
• NCBI Protein Information: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; tRNA-yW synthesizing protein 1 homolog A; tRNA wybutosine-synthesizing protein 1 homolog; radical S-adenosyl methionine and flavodoxin domains 1; radical S-adenosyl methionine and flavodoxin domain-containing protein 1
• UniProt Protein Name: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
• UniProt Gene Name: TYW1
• UniProt Synonym Gene Names: RSAFD1
• UniProt Entry Name: TYW1_HUMAN

NCBI Description

Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. [provided by RefSeq, Dec 2012]

Uniprot Description

TYW1: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Belongs to the TYW1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; RNA processing; EC 4.1.3.44

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: FMN binding; 4 iron, 4 sulfur cluster binding; iron ion binding; lyase activity; oxidoreductase activity

Biological Process: tRNA processing