VNN1 recombinant protein

VNN1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123050

• Gene Names: Vnn1; V-1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: VNN1; VNN1 Protein; Mouse; Recombinant (His Tag); Mouse VNN1/Vanin-1 Protein (His Tag); V-1 Protein; vanin 1; VNN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser487

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse VNN1 (Q9Z0K8)(Met 1-Ser 487), without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VNN1 recombinant protein

Background: Pantetheinase, also known as Pantetheine hydrolase, Vascular non-inflammatory molecule 1, Vanin-1, and VNN1, is a cell membrane protein which belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 contains one CN hydrolase domain. It is widely expressed with higher expression in spleen, kidney and blood. It is overexpressed in lesional psoriatic skin. Vanin-1 is also a member of the Vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sublethal irradiation.

References

Aurrand-Lions M, et al. (1996) Vanin-1, a Novel GPI-Linked Perivascular Molecule Involved in Thymus Homing. Immunity. 5 (5): 391-405.Grimmond S, et al. (2000) Sexually dimorphic expression of protease nexin-1 and vanin-1 in the developing mouse gonad prior to overt differentiation suggests a role in mammalian sexual development. Hum Mol Genet. 9 (10): 1553-60.Meghari S, et al. (2007) Vanin-1 controls granuloma formation and macrophage polarization in Coxiella burnetii infection. Eur J Immunol. 37 (1): 24-32.

NCBI and Uniprot Product Information

• NCBI GI #: 251823855
• NCBI GeneID: 22361
• NCBI Accession #: NP_035834.2
• NCBI GenBank Nucleotide #: NM_011704.3
• UniProt Accession #: Q9Z0K8; Q3TJI0; Q8VCT1; Q9DCH8
• Molecular Weight: 57,091 Da
• NCBI Official Full Name: pantetheinase
• NCBI Official Synonym Full Names: vanin 1
• NCBI Official Symbol: Vnn1
• NCBI Official Synonym Symbols: V-1
• NCBI Protein Information: pantetheinase
• UniProt Protein Name: Pantetheinase
• Protein Family: Pantetheinase
• UniProt Gene Name: Vnn1
• UniProt Synonym Gene Names: Vanin-1

Uniprot Description

Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.