5',3'-Nucleotidase Recombinant Protein | NT5M recombinant protein

Recombinant Human 5',3'-Nucleotidase, Mitochondrial

Cat. Num. AAA145401

• Gene Names: NT5M; mdN; dNT2; dNT-2
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: 5'; 3'-Nucleotidase; Recombinant Human 5'; Mitochondrial; NT5M Human; Mitochondrial Human Recombinant; 5'(3')-deoxyribonucleotidase mitochondrial; 3'-nucleotidase; mitochondrial; Deoxy-5'-nucleotidase 2; dNT-2; NT5M; DNT2; NT5M recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: NT5M protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC
• Sequence Length: 228

Related Product Information for NT5M recombinant protein

Description: NT5M Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 a.a.) and having a molecular mass of 25.1kDa.NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: NT5M is a member of the 5'(3')-deoxyribonucleotidase family. NT5M localizes to the mitochondrial matrix. The NT5M enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The NT5M gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unidentified etiology.

Product Categories/Family for NT5M recombinant protein

Enzymes; Nucleotidase

NCBI and Uniprot Product Information

• NCBI GI #: 9910372
• NCBI GeneID: 56953
• NCBI Accession #: NP_064586.1
• NCBI GenBank Nucleotide #: NM_020201.3
• UniProt Accession #: Q9NPB1
• Molecular Weight: 25,862 Da
• NCBI Official Full Name: 5'(3')-deoxyribonucleotidase, mitochondrial
• NCBI Official Synonym Full Names: 5',3'-nucleotidase, mitochondrial
• NCBI Official Symbol: NT5M
• NCBI Official Synonym Symbols: mdN; dNT2; dNT-2
• NCBI Protein Information: 5'(3')-deoxyribonucleotidase, mitochondrial; 5'(3')-deoxyribonucleotidase, mitochondrial; 5' nucleotidase, mitochondrial; 5(3)-deoxyribonucleotidase; deoxy-5'-nucleotidase 2; mitochondrial 5' nucleotidase
• UniProt Protein Name: 5'(3')-deoxyribonucleotidase, mitochondrial
• UniProt Gene Name: NT5M
• UniProt Synonym Gene Names: DNT2; 5',3'-nucleotidase, mitochondrial; dNT-2
• UniProt Entry Name: NT5M_HUMAN

NCBI Description

This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

NT5M: Dephosphorylates specifically the 5' and 2'(3')- phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP. Belongs to the 5'(3')-deoxyribonucleotidase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine; Mitochondrial; EC 3.1.3.-; Nucleotide Metabolism - purine; Phosphatase (non-protein); DNA replication

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding; nucleotidase activity

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine deoxyribonucleotide catabolic process; dUMP catabolic process; DNA replication