Pyruvate Kinase, Liver and RBC Active Protein | PKLR active protein

Recombinant Human Pyruvate Kinase, Liver and RBC

Cat. Num. AAA144279

• Gene Names: PKLR; PK1; PKL; PKR; RPK; PKRL
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Pyruvate Kinase; Liver and RBC; Recombinant Human Pyruvate Kinase; PKLR Human; Liver and RBC Human Recombinant; PK1; PKL; RPK; pyruvate kinase isozyme R/L; Red cell/liver pyruvate kinase; PKRL; PKLR active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The PKLR protein solution (1mg/1ml) contains 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 0.2M NaCl and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
• Sequence Length: 574

• Biological Activity: Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for PKLR active protein

Description: PKLR Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 549 amino acids (47-574a.a.) and having a molecular wieght of 59.2kDa. The PKLR is fused to 21a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.

Product Categories/Family for PKLR active protein

Protein Kinases; Enzymes; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 10835121
• NCBI GeneID: 5313
• NCBI Accession #: NP_000289.1
• NCBI GenBank Nucleotide #: NM_000298.5
• UniProt Accession #: P30613; O75758; P11973
• Molecular Weight: 58,494 Da
• NCBI Official Full Name: pyruvate kinase PKLR isoform 1
• NCBI Official Synonym Full Names: pyruvate kinase, liver and RBC
• NCBI Official Symbol: PKLR
• NCBI Official Synonym Symbols: PK1; PKL; PKR; RPK; PKRL
• NCBI Protein Information: pyruvate kinase PKLR; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; red cell/liver pyruvate kinase
• UniProt Protein Name: Pyruvate kinase PKLR
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKLR
• UniProt Synonym Gene Names: PK1; PKL
• UniProt Entry Name: KPYR_HUMAN

NCBI Description

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.

Protein type: EC 2.7.1.40; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytosol

Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: pyruvate biosynthetic process; glycolysis; response to cAMP; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; pathogenesis; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; energy reserve metabolic process; response to glucose stimulus; response to hypoxia; phosphorylation; response to nutrient

Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells