Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: 231 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Rabbit anti-Human PKLR Polyclonal Antibody | anti-PKLR antibody
PKLR Antibody
Immunohistochemistry: 1:20-1:100
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: 231 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: 231 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20. (Original magnification: ¡200))
NCBI and Uniprot Product Information
Uniprot Description
PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 2.7.1.40; Kinase, other; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; cytosol
Molecular Function: pyruvate kinase activity
Biological Process: cellular response to insulin stimulus; glycolysis
Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells
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Product Notes
The PKLR pklr (Catalog #AAA9416167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKLR Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKLR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:20-1:100. Researchers should empirically determine the suitability of the PKLR pklr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKLR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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