PKLR active protein

PKLR, 47-574aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203524

• Gene Names: PKLR; PK1; PKL; PKR; RPK; PKRL
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PKLR; 47-574aa; Human; His tag; E Coli (Bioactivity Validated); Pyruvate kinase isozymes R/L; PK1; PKL; PKR; PKRL; RPK; PKLR active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
• Sequence Length: 574

• Applicable Applications for PKLR active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity: > 80 units/mg. One unit will convert 1.0 umole of phospho (enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PKLR active protein

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PKLR active protein

Cancer

References

Meza NW., et al. (2009) Mol Ther. 17 (12):2000-9.; Roy MF., et al. (2007) J Exp Med. 204 (12):2949-61.;

NCBI and Uniprot Product Information

• NCBI GI #: 10835121
• NCBI GeneID: 5313
• NCBI Accession #: NP_000289
• NCBI GenBank Nucleotide #: NM_000298.5
• UniProt Accession #: P30613; O75758; P11973
• Molecular Weight: 59.2 kDa (549aa), confirmed by MALDI-TOF
• NCBI Official Full Name: pyruvate kinase PKLR isoform 1
• NCBI Official Synonym Full Names: pyruvate kinase L/R
• NCBI Official Symbol: PKLR
• NCBI Official Synonym Symbols: PK1; PKL; PKR; RPK; PKRL
• NCBI Protein Information: pyruvate kinase PKLR
• UniProt Protein Name: Pyruvate kinase PKLR
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKLR
• UniProt Synonym Gene Names: PK1; PKL

NCBI Description

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a key role in glycolysis.