Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using DNMT3L antibody.)
Rabbit anti-Human DNMT3L Polyclonal Antibody | anti-DNMT3L antibody
DNMT3L Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
IP: 1:20 - 1:50
ChIP: 1:20 - 1:50
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using DNMT3L antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using DNMT3L antibody.)
NCBI and Uniprot Product Information
NCBI Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: nucleus; cytosol
Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; DNA methylation on cytosine
Research Articles on DNMT3L
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Product Notes
The DNMT3L (Catalog #AAA127365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNMT3L Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT3L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP. WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:20 - 1:50 IP: 1:20 - 1:50 ChIP: 1:20 - 1:50. Researchers should empirically determine the suitability of the DNMT3L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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