Rabbit VANGL1 Polyclonal Antibody | anti-VANGL1 antibody

VANGL1 Polyclonal Antibody

Cat. Num. AAA3007630

• Gene Names: VANGL1; LPP2; STB2; STBM2; KITENIN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: VANGL1; Polyclonal Antibody; VANGL1 Polyclonal Antibody; Vang-like protein 1; Loop-tail protein 2 homolog; LPP2; Strabismus 2; Van Gogh-like protein 1; STB2; anti-VANGL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VANGL1 polyclonal antibody detects endogenous levels of VANGL1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 522

• Applicable Applications for anti-VANGL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human VANGL1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of VANGL1 polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:SK-OVCAR3 whole cell lysate (40ug))

Related Product Information for anti-VANGL1 antibody

Van Gogh-like proteins (VANGL1, VANGL2) are human orthologs of Drosophila Van Gogh (Vang/Stbm), a multi-pass transmembrane protein that is required to establish cell polarity in embryonic eyes, legs, and bristles. As in Drosophila, mammalian VANGL proteins are core components of the planar cell polarity (PCP) pathway that promotes asymmetric orientation of cells across a planar surface, and drives convergence-extension movements that are critical for tissue morphogenesis. Mutations in the human VANGL1 gene have been identified in patients identify with neural tube defects (e.g., spina bifida), providing evidence that VANGL1 plays a role in human embryonic morphogenesis. These findings are supported by genetic studies in mice, where mutations in both Vangl1 and Vangl2 result in neural tube defects. A possible role for VANGL in tumor progression is suggested by an increased expression of VANGL1 mRNA in breast cancer patients with an elevated risk of relapse.

NCBI and Uniprot Product Information

• NCBI GI #: 289547194
• NCBI GeneID: 81839
• NCBI Accession #: NP_001165882.1
• NCBI GenBank Nucleotide #: NP_001165882.1
• UniProt Accession #: Q8TAA9
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: vang-like protein 1 isoform 2
• NCBI Official Synonym Full Names: VANGL planar cell polarity protein 1
• NCBI Official Symbol: VANGL1
• NCBI Official Synonym Symbols: LPP2; STB2; STBM2; KITENIN
• NCBI Protein Information: vang-like protein 1
• UniProt Protein Name: Vang-like protein 1
• Protein Family: Vang-like protein
• UniProt Gene Name: VANGL1
• UniProt Synonym Gene Names: STB2; LPP2
• UniProt Entry Name: VANG1_HUMAN

NCBI Description

This gene encodes a member of the tretraspanin family. The encoded protein may be involved in mediating intestinal trefoil factor induced wound healing in the intestinal mucosa. Mutations in this gene are associated with neural tube defects. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

VANGL1: Defects in VANGL1 are a cause of neural tube defects (NTD). NTD are congenital malformations. The most common forms of NTD are described as open defects (including anencephaly and myelomeningocele, or spina bifida), which result from the failure of fusion in the cranial and spinal region of the neural tube, respectively. Other open dysraphisms (including myeloschisis, hemimyelomeningocele, and hemimyelocele) are sometimes associated with a Chiari type 2 malformation. A number of skin-covered (closed) NTD are categorized clinically depending on the presence of a subcutaneous mass (lipomyeloschisis, lipomyelomeningocele, meningocele, and myelocystocele) or the absence of such a mass (complex dysraphic states, including split cord malformations, dermal sinus, caudal regression, and segmental spinal dysgenesis). Defects in VANGL1 are a cause of sacral defect with anterior meningocele (SDAM). SDAM is a form of caudal dysgenesis. It is present at birth and becomes symptomatic later in life, usually because of obstructive labor in females, chronic constipation, or meningitis. Inheritance is autosomal dominant. Belongs to the Vang family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; lateral plasma membrane

Molecular Function: protein binding

Biological Process: multicellular organismal development

Disease: Neural Tube Defects; Sacral Defect With Anterior Meningocele