Canine 5-Hydroxytryptamine ELISA Kit | 5HT elisa kit

Canine 5-Hydroxytryptamine (5HT) ELISA Kit

Cat. Num. AAA283892

• Gene Names: HTR3E; 5-HT3E; 5-HT3-E; 5-HT3c1
• Reactivity: Canine
• Synonyms: 5-Hydroxytryptamine; Canine 5-Hydroxytryptamine (5HT) ELISA Kit; 5HT elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Canine 5HT. No significant cross-reactivity or interference between Canine 5HT and analogues was observed.
• Sequence Length: 456

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for 5HT elisa kit

Serotonin also called 5-hydroxytryptamine, is primarily found in the gastrointestinal (GI) tract and central nervous system (CNS) of animals. Approximately 80 percent of the human body's total serotonin is located in the enterochromaffin cells in the gut, where it is used to regulate intestinal movements. The remainder is synthesized in serotonergic neurons in the CNS where it has various functions, including the regulation of mood, appetite, sleep, muscle contraction, and some cognitive functions including memory and learning; and in blood platelets where it helps to regulate hemostasis and blood clotting. In addition to animals, serotonin is also found in fungi and plants. 5-hydroxytryptamine(5-HT) is an important neurotransmitter made by neurons in the central nervous system. These functions are mediated by a large number of receptors.After it is released by a neuron, 5-HT activates receptors located on nearby neurons.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate 5HT in samples. An antibody specific for 5HT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5HT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5HT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5HT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 375298705
• NCBI GeneID: 285242
• NCBI Accession #: NP_001243542.1
• NCBI GenBank Nucleotide #: NM_001256613.1
• Molecular Weight: 53,700 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 3E isoform d
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 3E
• NCBI Official Symbol: HTR3E
• NCBI Official Synonym Symbols: 5-HT3E; 5-HT3-E; 5-HT3c1
• NCBI Protein Information: 5-hydroxytryptamine receptor 3E
• UniProt Protein Name: 5-hydroxytryptamine receptor 3E
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR3E
• UniProt Synonym Gene Names: 5-HT3-E; 5-HT3E
• UniProt Entry Name: 5HT3E_HUMAN

NCBI Description

This locus encodes a 5-hydroxytryptamine (serotonin) receptor subunit. The encoded protein, subunit E, may play a role in neurotransmission in myenteric neurons. Genes encoding subunits C, D and E form a cluster on chromosome 3. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

5-HT(3E): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Channel, ligand-gated; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; plasma membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; protein binding; serotonin receptor activity; serotonin-activated cation-selective channel activity

Biological Process: serotonin receptor signaling pathway; synaptic transmission, cholinergic; transmembrane transport