Rabbit anti-Human TIP30 / HTATIP2 Polyclonal Antibody | anti-TIP30 antibody

Rabbit Polyclonal (IgG) to Human TIP30 / HTATIP2

Cat. Num. AAA248101

• Gene Names: HTATIP2; CC3; TIP30; SDR44U1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Immunoaffinity Purified
• Synonyms: TIP30 / HTATIP2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TIP30 / HTATIP2; Anti-TIP30 / HTATIP2 Antibody IHC-plus; HTATIP2; CC3; SDR44U1; Tat-interacting protein (30kD); TIP30; Oxidoreductase HTATIP2; Human TIP30; anti-TIP30 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human HTATIP2 / TIP30
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 242

• Applicable Applications for anti-TIP30 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC-P (5 ug/ml), IP (1:100 - 1:500), WB (1:1000 - 1:10000); Usage: Suggested starting dilutions are as follows: Western blot: 1:1000-1:10000, IHC-Paraffin: 5 ug/ml [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not...
• Target Species: Human
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 1 and 242 of TIP30 (SwissProt Q9BUP3).
• Immunogen Type: Recombinant protein
• Immunogen: TIP30 / HTATIP2 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 242 of TIP30 (SwissProt Q9BUP3).
• Preparation and Storage: Aliquot and freeze at -20 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TIP30 / HTATIP2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE HTATIP2 / TIP30 antibody diluted at 1:5000)

Immunoprecipitation (IP)

(IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody diluted at 1:1000.)

NCBI and Uniprot Product Information

• NCBI GI #: 148728164
• NCBI GeneID: 10553
• NCBI Accession #: NP_006401.3
• NCBI GenBank Nucleotide #: NM_006410.4
• UniProt Accession #: Q9BUP3; O15383; O60520; O95345; Q53GC1; Q53GG2; Q6IBI3; A8K7S7; D3DQY8
• Molecular Weight: 30,131 Da
• NCBI Official Full Name: oxidoreductase HTATIP2 isoform b
• NCBI Official Synonym Full Names: HIV-1 Tat interactive protein 2, 30kDa
• NCBI Official Symbol: HTATIP2
• NCBI Official Synonym Symbols: CC3; TIP30; SDR44U1
• NCBI Protein Information: oxidoreductase HTATIP2; Tat-interacting protein (30kD); HIV-1 TAT-interactive protein 2; 30 kDa HIV-1 TAT-interacting protein; short chain dehydrogenase/reductase family 44U, member 1
• UniProt Protein Name: Oxidoreductase HTATIP2
• UniProt Gene Name: HTATIP2
• UniProt Entry Name: HTAI2_HUMAN

Uniprot Description

HTATIP2: Oxidoreductase required for tumor suppression. NAPDH- bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transcription factor; Oxidoreductase; Nuclear envelope; EC 1.1.1.-; Tumor suppressor

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: membrane; cytoplasm; nuclear envelope; nucleus

Molecular Function: protein binding; transcription coactivator activity; oxidoreductase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; viral reproduction; apoptosis; nuclear import; angiogenesis; cell differentiation; regulation of angiogenesis; negative regulation of apoptosis