Human CTPS1 (CTP synthase 1) ELISA Kit | CTPS1 elisa kit

Human CTPS1 (CTP synthase 1) ELISA Kit

Cat. Num. AAA7612221

• Gene Names: CTPS1; CTPS; IMD24
• Reactivity: Human
• Synonyms: CTPS1 (CTP synthase 1); Human CTPS1 (CTP synthase 1) ELISA Kit; CTP synthetase 1; UTP-ammonia ligase 1; CTPS; CTPS1; CTPS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTPS1. No significant cross-reactivity or interference between CTPS1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTPS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20981706
• NCBI GeneID: 1503
• UniProt Accession #: P17812; Q5VW67; Q96GK6; B4DR64; D3DPW1
• Molecular Weight: 591
• NCBI Official Full Name: CTP synthase 1
• NCBI Official Synonym Full Names: CTP synthase 1
• NCBI Official Symbol: CTPS1
• NCBI Official Synonym Symbols: CTPS; IMD24
• NCBI Protein Information: CTP synthase 1; CTP synthetase 1; UTP--ammonia ligase 1; cytidine 5'-triphosphate synthetase; cytidine 5-prime triphosphate synthetase
• UniProt Protein Name: CTP synthase 1
• Protein Family: CTP synthase
• UniProt Gene Name: CTPS1
• UniProt Entry Name: PYRG1_HUMAN

NCBI Description

This gene encodes an enzyme responsible for the catalytic conversion of UTP (uridine triphosphate) to CTP (cytidine triphospate). This reaction is an important step in the biosynthesis of phospholipids and nucleic acids. Activity of this proten is important in the immune system, and loss of function of this gene has been associated with immunodeficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

CTPS: the enzyme cytidine-5-prime-triphosphate synthetase converts UTP to CTP. Important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis. The region to which the CTPS gene has been mapped is the location of breakpoints involved in several tumor types.

Protein type: Motility/polarity/chemotaxis; EC 6.3.4.2; Nucleotide Metabolism - pyrimidine; Ligase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: membrane; cytosol

Molecular Function: CTP synthase activity; ATP binding

Biological Process: T cell proliferation; B cell proliferation; response to drug; CTP biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; glutamine metabolic process

Disease: Immunodeficiency 24