Rabbit anti-Human 5HT3E Polyclonal Antibody | anti-HTR3E antibody

5HT3E Antibody (Center)

Cat. Num. AAA9208743

• Gene Names: HTR3E; 5-HT3E; 5-HT3-E; 5-HT3c1
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: 5HT3E; Polyclonal Antibody; 5HT3E Antibody (Center); 5-hydroxytryptamine receptor 3E; 5-HT3-E; 5-HT3E; Serotonin receptor 3E; HTR3E; anti-HTR3E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This 5HT3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the Central region of human 5HT3E.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 126-154
• Sequence Length: 482

• Applicable Applications for anti-HTR3E antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(5HT3E Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the 5HT3E antibody detected the 5HT3E protein (arrow).)

Flow Cytometry (FC/FACS)

(5HT3E Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HTR3E antibody

The product of this gene belongs to the ligand-gated ion
channel receptor superfamily. HTR3E encodes a subunit E of the
type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic
hormone that functions as a neurotransmitter, a hormone, and a
mitogen. This receptor causes fast, depolarizing responses in
neurons after activation. Genes encoding subunits C, D and E form a
cluster on chromosome 3. An alternative splice variant has been
described but its full length sequence has not been determined.

Product Categories/Family for anti-HTR3E antibody

Metabolism; Neuroscience; Signal Transduction

References

Walstab, J., et al. J. Biol. Chem. 285(35):26956-26965(2010)
Hammer, C., et al. Pharmacogenomics 11(7):943-950(2010)
Lennertz, L., et al. Eur Neuropsychopharmacol 20(6):414-420(2010)
Goecke, T.W., et al. Acta Obstet Gynecol Scand 89(1):7-14(2010)
Schuhmacher, A., et al. Pharmacogenet. Genomics 19(11):843-851(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 375298705
• NCBI GeneID: 285242
• NCBI Accession #: NP_001243542.1; NP_001243543.1; NP_872395.2; NP_938055.1; NP_938056.1
• NCBI GenBank Nucleotide #: NM_001256613.1
• UniProt Accession #: A5X5Y0; Q495G1; Q495G3; Q6V706; Q6V707; Q7Z6B2; A8IKD7; E9PGF1
• Molecular Weight: 51438
• NCBI Official Full Name: 5-hydroxytryptamine receptor 3E isoform d
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 3E, ionotropic
• NCBI Official Symbol: HTR3E
• NCBI Official Synonym Symbols: 5-HT3E; 5-HT3-E; 5-HT3c1
• NCBI Protein Information: 5-hydroxytryptamine receptor 3E
• UniProt Protein Name: 5-hydroxytryptamine receptor 3E
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR3E
• UniProt Synonym Gene Names: 5-HT3-E; 5-HT3E
• UniProt Entry Name: 5HT3E_HUMAN

NCBI Description

This locus encodes a 5-hydroxytryptamine (serotonin) receptor subunit. The encoded protein, subunit E, may play a role in neurotransmission in myenteric neurons. Genes encoding subunits C, D and E form a cluster on chromosome 3. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

5-HT(3E): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Channel, ligand-gated; Membrane protein, integral; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: extracellular ligand-gated ion channel activity

Biological Process: transmembrane transport