Bovine Protein Z Dependent Protease Inhibitor ELISA Kit | ZPI elisa kit

Bovine Protein Z Dependent Protease Inhibitor ELISA Kit

Cat. Num. AAA735327

• Gene Names: SERPINA10; PZI; ZPI
• Reactivity: Bovine
• Synonyms: Protein Z Dependent Protease Inhibitor; Bovine Protein Z Dependent Protease Inhibitor ELISA Kit; ZPI elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ZPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ZPI. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ZPI elisa kit

Principle of the assay: ZPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZPI antibody and an ZPI-HRP conjugate. The assay sample and buffer are incubated together with ZPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZPI concentration since ZPI from samples and ZPI-HRP conjugate compete for the anti-ZPI antibody binding site. Since the number of sites is limited, as more sites are occupied by ZPI from the sample, fewer sites are left to bind ZPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZPI concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 154759291
• NCBI GeneID: 51156
• NCBI Accession #: NP_001094077.1
• NCBI GenBank Nucleotide #: NM_001100607.2
• Molecular Weight: 50,707 Da
• NCBI Official Full Name: protein Z-dependent protease inhibitor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• NCBI Official Symbol: SERPINA10
• NCBI Official Synonym Symbols: PZI; ZPI
• NCBI Protein Information: protein Z-dependent protease inhibitor; serpin A10; PZ-dependent protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• UniProt Protein Name: Protein Z-dependent protease inhibitor
• UniProt Gene Name: SERPINA10
• UniProt Synonym Gene Names: ZPI; UNQ707/PRO1358; PZ-dependent protease inhibitor; PZI
• UniProt Entry Name: ZPI_HUMAN

NCBI Description

The protein encoded by this gene belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

SERPINA10: Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding

Biological Process: regulation of acrosome reaction; blood coagulation