Rabbit anti-Human ATP1B1 Polyclonal Antibody | anti-ATP1B1 antibody

ATP1B1, ID (ATP1B1, ATP1B, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1)

Cat. Num. AAA6008813

• Gene Names: ATP1B1; ATP1B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ATP1B1; Polyclonal Antibody; ID (ATP1B1; ATP1B; Sodium/potassium-transporting ATPase subunit beta-1; Sodium/potassium-dependent ATPase subunit beta-1); Anti -ATP1B1; anti-ATP1B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ATP1B1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ATP1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-146 amino acids from the Central region of human ATP1B1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATP1B1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ATP1B1 antibody detected the ATP1B1 protein (arrow).)

Immunohistochemistry (IHC)

(ATP1B1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP1B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ATP1B1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 47115169
• NCBI GeneID: 481
• UniProt Accession #: Q6LEU2; P05026
• Molecular Weight: 35,049 Da
• NCBI Official Full Name: ATP1B1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: ATP1B1
• NCBI Official Synonym Symbols: ATP1B
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; adenosinetriphosphatase; sodium pump subunit beta-1; Na, K-ATPase beta-1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase beta-1 chain; sodium-potassium ATPase subunit beta 1 (non-catalytic)
• UniProt Protein Name: ATP1B1 protein
• UniProt Gene Name: ATP1B1
• UniProt Entry Name: Q6LEU2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

Uniprot Description

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; vesicle; sarcolemma

Molecular Function: ATPase activator activity; protein binding; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular calcium ion homeostasis; cellular sodium ion homeostasis; potassium ion import; protein stabilization; positive regulation of ATPase activity; regulation of gene expression; response to hypoxia; blood coagulation; cell adhesion; cellular potassium ion homeostasis; leukocyte migration; transmembrane transport; cardiac muscle contraction

Disease: Hypertension, Essential