Rabbit HUS1 Polyclonal Antibody | anti-HUS1 antibody

HUS1 (hHUS1, Checkpoint Protein HUS1B hHUS1B) (HRP)

Cat. Num. AAA6421847

• Gene Names: HUS1; hHUS1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: HUS1; Polyclonal Antibody; HUS1 (hHUS1; Checkpoint Protein HUS1B hHUS1B) (HRP); anti-HUS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes HUS1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-HUS1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human HUS1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HUS1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using HUS1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-HUS1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4758576
• NCBI GeneID: 3364
• NCBI Accession #: NP_004498.1
• NCBI GenBank Nucleotide #: NM_004507.3
• UniProt Accession #: O60921; B4DFI9
• Molecular Weight: 29,287 Da
• NCBI Official Full Name: checkpoint protein HUS1
• NCBI Official Synonym Full Names: HUS1 checkpoint homolog (S. pombe)
• NCBI Official Symbol: HUS1
• NCBI Official Synonym Symbols: hHUS1
• NCBI Protein Information: checkpoint protein HUS1; hus1+-like protein
• UniProt Protein Name: Checkpoint protein HUS1
• Protein Family: Checkpoint protein
• UniProt Gene Name: HUS1
• UniProt Synonym Gene Names: hHUS1
• UniProt Entry Name: HUS1_HUMAN

NCBI Description

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Uniprot Description

HUS1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Belongs to the HUS1 family.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: embryonic development; regulation of protein amino acid phosphorylation; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA replication; DNA repair; protein amino acid phosphorylation; response to DNA damage stimulus; double-strand break repair via homologous recombination; response to UV