Typical Testing Data/Standard Curve (for reference only)
Rat Zona pellucida sperm-binding protein 3 ELISA Kit | Zp3 elisa kit
Rat Zona pellucida sperm-binding protein 3 ELISA Kit
Gene Names
Zp3; Zp-3
Reactivity
Rat
Synonyms
Zona pellucida sperm-binding protein 3; Rat Zona pellucida sperm-binding protein 3 ELISA Kit; Zona pellucida glycoprotein 3; Zp-3; Zona pellucida protein C; Zp3; Zpc; Zp3 elisa kit
Reactivity
Rat
Specificity
This assay recognizes recombinant and natural ratZona pellucida sperm-binding protein 3. No significant cross-reactivity or interference was observed.
Sequence Length
424
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.156 ng/mL
Intra-Assay CV
<=4.9%
Inter-Assay CV
<=7.6%
Recovery
95%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Zp3 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofratZona pellucida sperm-binding protein 3concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona pellucida sperm-binding protein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forZona pellucida sperm-binding protein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zona pellucida sperm-binding protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Zona pellucida sperm-binding protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona pellucida sperm-binding protein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forZona pellucida sperm-binding protein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zona pellucida sperm-binding protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Zona pellucida sperm-binding protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,901 Da
NCBI Official Full Name
zona pellucida sperm-binding protein 3
NCBI Official Synonym Full Names
zona pellucida glycoprotein 3 (sperm receptor)
NCBI Official Symbol
Zp3
NCBI Official Synonym Symbols
Zp-3
NCBI Protein Information
zona pellucida sperm-binding protein 3
UniProt Protein Name
Zona pellucida sperm-binding protein 3
Protein Family
UniProt Gene Name
Zp3
UniProt Synonym Gene Names
Zp-3; Zpc; Zp-3
UniProt Entry Name
ZP3_RAT
NCBI Description
glycoprotein component of the zona pellucida [RGD, Feb 2006]
Uniprot Description
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
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Product Notes
The Rat Zp3 zp3 (Catalog #AAA2880882) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880882 ELISA Kit recognizes Rat Zp3. It is sometimes possible for the material contained within the vial of "Zona pellucida sperm-binding protein 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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