Mouse Heat shock-related 70 kDa protein 2 ELISA Kit | Hspa2 elisa kit

Mouse Heat shock-related 70 kDa protein 2 ELISA Kit

Cat. Num. AAA2886655

• Gene Names: Hspa2; 70kDa; HSP70.2; HSP70A2; Hsp70-2
• Reactivity: Mouse
• Synonyms: Heat shock-related 70 kDa protein 2; Mouse Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock protein 70.2; Hspa2; Hcp70.2; Hsp70-2; Hspa2 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouseHeat shock protein 70.2. No significant cross-reactivity or interference was observed.
• Sequence Length: 633

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.156 ng/mL
• Intra-Assay CV: <=6.2%
• Inter-Assay CV: <=7.6%
• Recovery: 95%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hspa2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofmouseHeat shock protein 70.2concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat shock protein 70.2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHeat shock protein 70.2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock protein 70.2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Heat shock protein 70.2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50345978
• NCBI GeneID: 15512
• NCBI Accession #: NP_001002012.1
• NCBI GenBank Nucleotide #: NM_001002012.1
• UniProt Accession #: P17156; Q99KD7
• Molecular Weight: 69,642 Da
• NCBI Official Full Name: heat shock-related 70 kDa protein 2
• NCBI Official Synonym Full Names: heat shock protein 2
• NCBI Official Symbol: Hspa2
• NCBI Official Synonym Symbols: 70kDa; HSP70.2; HSP70A2; Hsp70-2
• NCBI Protein Information: heat shock-related 70 kDa protein 2
• UniProt Protein Name: Heat shock-related 70 kDa protein 2
• Protein Family: Heat shock-related 70 kDa protein
• UniProt Gene Name: Hspa2
• UniProt Synonym Gene Names: Hcp70.2; Hsp70-2; Heat shock protein 70.2
• UniProt Entry Name: HSP72_MOUSE

Uniprot Description

HSPA2: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Interacts with ZNF541. Component of the CatSper complex. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone

Cellular Component: cell surface; cytosol; male germ cell nucleus; membrane; mitochondrion; myelin sheath; nucleus; synaptonemal complex

Molecular Function: ATP binding; enzyme binding; glycolipid binding; nucleotide binding; protein binding; unfolded protein binding

Biological Process: G2/M-specific positive regulation of cyclin-dependent protein kinase activity; male meiosis; male meiosis I; positive regulation of ATPase activity; positive regulation of protein amino acid phosphorylation; protein refolding; spermatid development