Typical Testing Data/Standard Curve (for reference only)
Human Zona pellucida sperm binding protein 3 (ZP3) ELISA Kit | ZP3 elisa kit
Human Zona pellucida sperm binding protein 3 (ZP3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ZP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ZP3 antibody and an ZP3-HRP conjugate. The assay sample and buffer are incubated together with ZP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZP3 concentration since ZP3 from samples and ZP3-HRP conjugate compete for the anti-ZP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZP3 from the sample, fewer sites are left to bind ZP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZP3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ZP3: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Belongs to the ZP domain family. ZPC subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; acrosome; extracellular region; integral to membrane; secretory granule; extracellular matrix; multivesicular body; proteinaceous extracellular matrix; perinuclear region of cytoplasm; cytoplasm; plasma membrane
Molecular Function: acrosin binding; protein binding; signal transducer activity; store-operated calcium channel activity; manganese ion transmembrane transporter activity; carbohydrate binding
Biological Process: positive regulation of type IV hypersensitivity; phosphoinositide-mediated signaling; binding of sperm to zona pellucida; positive regulation of leukocyte migration; positive regulation of transcription, DNA-dependent; manganese ion transport; blastocyst formation; positive regulation of phosphatidylinositol biosynthetic process; multicellular organism reproduction; positive regulation of interleukin-4 production; humoral immune response mediated by circulating immunoglobulin; positive regulation of protein kinase B signaling cascade; intracellular protein transport; positive regulation of interferon-gamma production; positive regulation of humoral immune response; positive regulation of protein kinase activity; single fertilization; positive regulation of T cell proliferation; negative regulation of transcription, DNA-dependent; oocyte development; positive regulation of inflammatory response
Research Articles on ZP3
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Product Notes
The Human ZP3 zp3 (Catalog #AAA7235222) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235222 ELISA Kit recognizes Human ZP3. It is sometimes possible for the material contained within the vial of "Zona pellucida sperm binding protein 3 (ZP3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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