Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DmX-like protein 1 (DMXL1) Standard Curve.)
Mouse DmX-Like Protein 1 (DMXL1) ELISA Kit | DMXL1 elisa kit
Mouse DmX-Like Protein 1 (DMXL1) ELISA Kit
Gene Names
Dmxl1; D030030C22; C630007L23Rik
Reactivity
Mouse
Synonyms
DmX-Like Protein 1 (DMXL1); Mouse DmX-Like Protein 1 (DMXL1) ELISA Kit; DMXL1; DKFZp779O1239; FLJ44772; DMXL1 elisa kit
Reactivity
Mouse
Specificity
Mouse DmX-like protein 1 (DMXL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMXL1. No significant cross-reactivity or interference between Mouse DMXL1 and analogues was observed.
Sequence Length
3013
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DmX-like protein 1 (DMXL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DmX-like protein 1 (DMXL1) Standard Curve.)
Related Product Information for DMXL1 elisa kit
Description: This Mouse DmX-like protein 1 (DMXL1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMXL1 in samples. An antibody specific for DMXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By EST database searching for sequences showing homology to Drosophila DmX and by use of primers designed for RT-PCR from human placental mRNA, Kraemer et al. (2000) cloned a nearly full-length homolog, which they designated DMX-like-1. The DMXL1 cDNA encodes a deduced 3,027-amino acid protein with a calculated mass of approximately 338 kD. The protein belongs to the superfamily of WD repeat proteins, most of which have regulatory functions, and contains at least 28 WD repeat units. DMXL1 shows a high level of evolutionary conservation. By searching EST databases with the complete cDNA sequence as query, Kraemer et al. (2000) found that DMXL1 is expressed in a variety of tissues, including bone, breast, eye, foreskin, heart, parathyroid, small intestine, testis, tonsils, uterus, and whole embryo.
Background: By EST database searching for sequences showing homology to Drosophila DmX and by use of primers designed for RT-PCR from human placental mRNA, Kraemer et al. (2000) cloned a nearly full-length homolog, which they designated DMX-like-1. The DMXL1 cDNA encodes a deduced 3,027-amino acid protein with a calculated mass of approximately 338 kD. The protein belongs to the superfamily of WD repeat proteins, most of which have regulatory functions, and contains at least 28 WD repeat units. DMXL1 shows a high level of evolutionary conservation. By searching EST databases with the complete cDNA sequence as query, Kraemer et al. (2000) found that DMXL1 is expressed in a variety of tissues, including bone, breast, eye, foreskin, heart, parathyroid, small intestine, testis, tonsils, uterus, and whole embryo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dmX-like protein 1
NCBI Official Synonym Full Names
Dmx-like 1
NCBI Official Symbol
Dmxl1
NCBI Official Synonym Symbols
D030030C22; C630007L23Rik
NCBI Protein Information
dmX-like protein 1
UniProt Protein Name
DmX-like protein 1
Protein Family
UniProt Gene Name
Dmxl1
UniProt Synonym Gene Names
X-like 1 protein
UniProt Entry Name
DMXL1_MOUSE
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Product Notes
The Mouse DMXL1 dmxl1 (Catalog #AAA9717840) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717840 ELISA Kit recognizes Mouse DMXL1. It is sometimes possible for the material contained within the vial of "DmX-Like Protein 1 (DMXL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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