Mouse Zinc finger protein 148 ELISA Kit | ZNF148 elisa kit
Mouse Zinc finger protein 148 (ZNF148) ELISA Kit
Gene Names
Zfp148; BERF-1; BFCOL1; ZBP-89; Znf148; AI480666; AW045217; 2210405J08Rik
Reactivity
Mouse
Synonyms
Zinc finger protein 148; Mouse Zinc finger protein 148 (ZNF148) ELISA Kit; ZNF148 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZNF148. No significant cross-reactivity or interference between Mouse ZNF148 and analogues was observed.
Sequence Length
794
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZNF148 elisa kit
ZNF148 inhibited SP1 activation of the ornithine decarboxylase (ODC) gene. ZNF148 and SP1 bound to a GC box in the proximal promoter of the ODC gene in a mutually exclusive manner. ZNF148 also repressed SP1-mediated activation of the vimentin promoter. The N-terminal zinc fingers of ZNF148 interacted with SP1 and repressed gene expression, and cotransfection of TAFII130 (TAF4) with ZNF148 and SP1 in insect cells rescued expression.ZNF148 gene contains 9 exons and spans about 130 kb. Exon 4 contains the translation initiation site. Mouse Znf148 showed the same genomic structure with identical intron-exon organization, and the promoter region displayed typical features of a housekeeping gene, with a high G/C content and the absence of canonical TATA and CAAT boxes.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF148 in samples. An antibody specific for ZNF148 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF148 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF148 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF148 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF148 in samples. An antibody specific for ZNF148 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF148 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF148 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF148 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,751 Da
NCBI Official Full Name
zinc finger protein 148
NCBI Official Synonym Full Names
zinc finger protein 148
NCBI Official Symbol
Zfp148
NCBI Official Synonym Symbols
BERF-1; BFCOL1; ZBP-89; Znf148; AI480666; AW045217; 2210405J08Rik
NCBI Protein Information
zinc finger protein 148
UniProt Protein Name
Zinc finger protein 148
Protein Family
UniProt Gene Name
Znf148
UniProt Synonym Gene Names
Zbp89; Zfp148
Uniprot Description
ZNF148: a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 16 B3|16 23.93 cM
Cellular Component: Golgi apparatus; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding
Biological Process: gamete generation; negative regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZNF148
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Product Notes
The Mouse ZNF148 znf148 (Catalog #AAA281874) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281874 ELISA Kit recognizes Mouse ZNF148. It is sometimes possible for the material contained within the vial of "Zinc finger protein 148, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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