Replication Factor C5 (RFC5) Recombinant Protein | RFC5 recombinant protein

Recombinant Replication Factor C5 (RFC5)

Cat. Num. AAA2086872

• Gene Names: RFC5; RFC36
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Replication Factor C5 (RFC5); Recombinant Replication Factor C5 (RFC5); RFC36; Activator 1 36 kDa subunit; Activator 1 subunit 5; Replication factor C 36 kDa subunit; RFC5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 340

• Applicable Applications for RFC5 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Met1~Ile219
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 8.3
• Subcellular Location: Nucleus.
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for RFC5 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 6677723
• NCBI GeneID: 5985
• NCBI Accession #: NP_031396.1
• NCBI GenBank Nucleotide #: NM_007370.6
• UniProt Accession #: P40937; A8MZ62; B3KSX8
• Molecular Weight: 28.5kDa
• NCBI Official Full Name: replication factor C subunit 5 isoform 1
• NCBI Official Synonym Full Names: replication factor C subunit 5
• NCBI Official Symbol: RFC5
• NCBI Official Synonym Symbols: RFC36
• NCBI Protein Information: replication factor C subunit 5
• UniProt Protein Name: Replication factor C subunit 5
• Protein Family: Replication factor
• UniProt Gene Name: RFC5
• UniProt Synonym Gene Names: A1 36 kDa subunit; RF-C 36 kDa subunit; RFC36

NCBI Description

This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Uniprot Description

RFC5: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: DNA replication factor C complex; nucleoplasm

Molecular Function: DNA clamp loader activity; protein binding; single-stranded DNA-dependent ATPase activity

Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; error-prone postreplication DNA repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair