Human ribonucleoprotein K ELISA Kit | ZMAT1 elisa kit
Human Zinc finger protein interacting with ribonucleoprotein K (ZIK1) ELISA Kit
Gene Names
ZIK1; ZNF762
Reactivity
Human
Synonyms
ribonucleoprotein K; Human Zinc finger protein interacting with ribonucleoprotein K (ZIK1) ELISA Kit; ZIK1; GHc-311G7.1; KIAA1789; MGC176597; MGC176728; zinc finger matrin-type protein 1; ZMAT1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ZIK1. No significant cross-reactivity or interference between Human ZIK1 and analogues was observed.
Sequence Length
346
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZMAT1 elisa kit
Zik1 contains a KRAB-A domain thought to be involved in transcriptional repression. Zik1 and K protein bound in vitro and co-immunoprecipitated from cell extracts indicating that in vivo their interaction is direct. Expression of Gal4 DNA-binding domain-Zik1 fusion protein repressed a gene promoter bearing Gal4-binding elements, indicating that from cognate DNA elements Zik1 is a transcriptional repressor.
The known diverse nature of K protein molecular interactions and now the identification of a K protein partner that is a transcriptional repressor lends support to the notion that K protein is a remarkably versatile molecule that may be acting as a docking platform to facilitate communication among molecules involved in signal transduction and gene expression.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZIK1 in samples. An antibody specific for ZIK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZIK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZIK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZIK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The known diverse nature of K protein molecular interactions and now the identification of a K protein partner that is a transcriptional repressor lends support to the notion that K protein is a remarkably versatile molecule that may be acting as a docking platform to facilitate communication among molecules involved in signal transduction and gene expression.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZIK1 in samples. An antibody specific for ZIK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZIK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZIK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZIK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,718 Da
NCBI Official Full Name
zinc finger protein interacting with ribonucleoprotein K
NCBI Official Synonym Full Names
zinc finger protein interacting with K protein 1
NCBI Official Symbol
ZIK1
NCBI Official Synonym Symbols
ZNF762
NCBI Protein Information
zinc finger protein interacting with ribonucleoprotein K
UniProt Protein Name
Zinc finger protein interacting with ribonucleoprotein K
Protein Family
UniProt Gene Name
ZIK1
UniProt Synonym Gene Names
ZNF762
Uniprot Description
ZIK1: May be a transcriptional repressor. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 19q13.43
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The Human ZMAT1 zik1 (Catalog #AAA285320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285320 ELISA Kit recognizes Human ZMAT1. It is sometimes possible for the material contained within the vial of "ribonucleoprotein K, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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