Rabbit anti-Mouse PIK3C2A Polyclonal Antibody | anti-PIK3C2A antibody

PIK3C2A, CT (PIK3C2A, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha) (Biotin)

Cat. Num. AAA6333972

• Gene Names: PIK3C2A; CPK; PI3-K-C2A; PI3-K-C2(ALPHA)
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIK3C2A; Polyclonal Antibody; CT (PIK3C2A; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; Phosphoinositide 3-kinase-C2-alpha) (Biotin); Phosphoinositide 3-kinase-C2-alpha; anti-PIK3C2A antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PIK3C2A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PIK3C2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1438-1466 amino acids from the C-terminal region of human PIK3C2A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIK3C2A Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PIK3C2A antibody detected the PIK3C2A protein (arrow).)

Immunohistochemistry (IHC)

(PIK3C2A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3C2A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PIK3C2A antibody

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Product Categories/Family for anti-PIK3C2A antibody

Antibodies; Protein Kinases

References

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kostakis, G.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109 (5), E53-E58 (2010): Koutros, S., et al. Cancer Res. 70(6):2389-2396(2010). Ng, S.K., et al. Biochem. Biophys. Res. Commun. 387(2):310-315(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 157671929
• NCBI GeneID: 5286
• NCBI Accession #: NP_002636.2
• NCBI GenBank Nucleotide #: NM_002645.2
• UniProt Accession #: O00443; Q14CQ9; B0LPH2; B4E2G4
• Molecular Weight: 53,742 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
• NCBI Official Symbol: PIK3C2A
• NCBI Official Synonym Symbols: CPK; PI3-K-C2A; PI3-K-C2(ALPHA)
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; PI3K-C2alpha; PI3K-C2-alpha; ptdIns-3-kinase C2 subunit alpha; phosphoinositide 3-kinase-C2-alpha; C2-containing phosphatidylinositol kinase; phosphoinositide-3-kinase, class 2,
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• Protein Family: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
• UniProt Gene Name: PIK3C2A
• UniProt Synonym Gene Names: PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha
• UniProt Entry Name: P3C2A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.

Protein type: Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; Motility/polarity/chemotaxis; Autophagy; EC 2.7.1.154

Chromosomal Location of Human Ortholog: 11p15.5-p14

Cellular Component: Golgi apparatus; membrane; clathrin-coated vesicle; cytoplasm; plasma membrane; nucleus; cytosol; phosphoinositide 3-kinase complex; vesicle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; phosphoinositide binding; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; phosphoinositide 3-kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; clathrin cage assembly; exocytosis; phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; vascular smooth muscle contraction; insulin receptor signaling pathway; endocytosis; platelet-derived growth factor receptor signaling pathway