14-3-3 beta Recombinant Protein | YWHAB recombinant protein

14-3-3 beta Protein, Cynomolgus, Recombinant

Cat. Num. AAA8123525

• Gene Names: YWHAB; GW128
• Purity: >90% as determined by SDS-PAGE
• Synonyms: 14-3-3 beta; 14-3-3 beta Protein; Cynomolgus; Recombinant; Cynomolgus 14-3-3 beta/YWHAB Protein; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta; YWHAB recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol, 0.5mM DTT, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met2-Asn244

• Species: Cynomolgus
• Predicted N Terminal: Met 1
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the mature form of Cynomolgus (Macaca fascicularis) YWHAB (Q4R572-2)(Met 2-Asn 244) was expressed and purified, with an initial Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for YWHAB recombinant protein

Background: 14-3-3 beta/YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta/YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta/YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta/YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta/YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Product Categories/Family for YWHAB recombinant protein

phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins; Death Receptor Signaling

References

Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.

NCBI and Uniprot Product Information

• NCBI GI #: 544465942
• NCBI GeneID: 101926442
• NCBI Accession #: XP_005569168.1
• NCBI GenBank Nucleotide #: XM_005569111.2
• UniProt Accession #: Q4R572
• Molecular Weight: 27,850 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: GW128
• NCBI Protein Information: 14-3-3 protein beta/alpha
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAB

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.