14-3-3 epsilon Recombinant Protein | YWHAE recombinant protein

14-3-3 epsilon Protein, Mouse, Recombinant (GST Tag)

Cat. Num. AAA8122915

• Gene Names: Ywhae; AU019196
• Purity: >90% as determined by SDS-PAGE
• Synonyms: 14-3-3 epsilon; 14-3-3 epsilon Protein; Mouse; Recombinant (GST Tag); Mouse 14-3-3 epsilon/YWHAE Protein (GST Tag); AU019196 Protein; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; epsilon; YWHAE recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 2mM GSH, 1mM DTT, pH8.2. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln255

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mouse YWHAE (P62259)(Met 1-Gln 255) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for YWHAE recombinant protein

Background: YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon/YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon/YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon/YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon/YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Product Categories/Family for YWHAE recombinant protein

phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins; Death Receptor Signaling

References

Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.

NCBI and Uniprot Product Information

• NCBI GI #: 226874906
• NCBI GeneID: 22627
• NCBI Accession #: NP_033562.3
• NCBI GenBank Nucleotide #: NM_009536.4
• UniProt Accession #: P62259; P29360; P42655; Q63631
• Molecular Weight: 22.2 kDa
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• NCBI Official Symbol: Ywhae
• NCBI Official Synonym Symbols: AU019196
• NCBI Protein Information: 14-3-3 protein epsilon
• UniProt Protein Name: 14-3-3 protein epsilon
• Protein Family: 14-3-3 protein
• UniProt Gene Name: Ywhae
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_MOUSE

Uniprot Description

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Cellular Component: kinesin complex; focal adhesion; mitochondrion; membrane; axon; cytoplasm

Molecular Function: protein domain specific binding; protein binding; enzyme binding; potassium channel regulator activity; protein heterodimerization activity; histone deacetylase binding; protein complex binding; phosphoprotein binding; phosphoserine binding

Biological Process: negative regulation of protein amino acid dephosphorylation; hippocampus development; neuron migration; cerebral cortex development; protein targeting