Western Blot (WB)
(Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Hep G2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
Rabbit anti-Human 14-3-3 beta Polyclonal Antibody | anti-YWHAB antibody
Anti-14-3-3 beta Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
14-3-3 beta; Polyclonal Antibody; Anti-14-3-3 beta Antibody; Rabbit Polyclonal; 14-3-3 beta Antibody; Rabbit PAb; Antigen Affinity Purified; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta; Anti-GW128 Antibody; Anti-HEL-S-1 Antibody; Anti-HS1 Antibody; Anti-KCIP-1 Antibody; Anti-YWHAA Antibody; anti-YWHAB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human 14-3-3 beta
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-YWHAB antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human 14-3-3 beta fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human 14-3-3 beta fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Hep G2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
Western Blot (WB)
(Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Hep G2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
Immunoprecipitation (IP)
(YWHAB was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 4 uL anti-YWHAB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-YWHAB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size :28 kDa)
Immunoprecipitation (IP)
(YWHAB was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 4 uL anti-YWHAB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-YWHAB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size :28 kDa)
Related Product Information for anti-YWHAB antibody
14-3-3 beta/YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta/YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta/YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta/YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta/YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
Product Categories/Family for anti-YWHAB antibody
References
Tommerup N, et al. (1996) Assignment of the human genes encoding 14, 3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
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Product Notes
The YWHAB (Catalog #AAA8108173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-14-3-3 beta Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the YWHAB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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