X-ray repair cross-complementing protein 6 (Xrcc6) Recombinant Protein | Xrcc6 recombinant protein

Recombinant Mouse X-ray repair cross-complementing protein 6 (Xrcc6)

Cat. Num. AAA1264976

• Gene Names: Xrcc6; Ku70; 70kDa; G22p1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: X-ray repair cross-complementing protein 6 (Xrcc6); Recombinant Mouse X-ray repair cross-complementing protein 6 (Xrcc6); Xrcc6 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-608, Full length protein
• Sequence: SEWESYYKTEGEEEEEEEESPDTGGEYKYSGRDSLIFLVDASRAMFESQGEDELTPFDMSIQCIQSVYTSKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQDLDNPGAKRVLELDQFKGQQGKKHFRDTVGHGSDYSLSEVLWVCANLFSDVQLKMSHKRIMLFTNEDDPHGRDSAKASRARTKASDLRDTGIFLDLMHLKKPGGFDVSVFYRDIITTAEDEDLGVHFEESSKLEDLLRKVRAKETKKRVLSRLKFKLGEDVVLMVGIYNLVQKANKPFPVRLYRETNEPVKTKTRTFNVNTGSLLLPSDTKRSLTYGTRQIVLEKEETEELKRFDEPGLILMGFKPTVMLKKQHYLRPSLFVYPEESLVSGSSTLFSALLTKCVEKKVIAVCRYTPRKNVSPYFVALVPQEEELDDQNIQVTPGGFQLVFLPYADDKRKVPFTEKVTANQEQIDKMKAIVQKLRFTYRSDSFENPVLQQHFRNLEALALDMMESEQVVDLTLPKVEAIKKRLGSLADEFKELVYPPGYNPEGKVAKRKQDDEGSTSKKPKVELSEEELKAHFRKGTLGKLTVPTLKDICKAHGLKSGPKKQELLDALIRHLEKN
• Sequence Length: 607

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 145587104
• NCBI GeneID: 14375
• NCBI Accession #: NP_034377.2
• NCBI GenBank Nucleotide #: NM_010247.2
• UniProt Accession #: P23475; O88212; Q3UJL8; Q62027; Q62382; Q62453; Q6GTV8; Q8QZX7
• Molecular Weight: 69,484 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 6
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 6
• NCBI Official Symbol: Xrcc6
• NCBI Official Synonym Symbols: Ku70; 70kDa; G22p1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: X-ray repair cross-complementing protein
• UniProt Gene Name: Xrcc6
• UniProt Synonym Gene Names: G22p1; Ku70; 5'-dRP/AP lyase Ku70; CTC75; CTCBF; Ku70

Uniprot Description

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway ().