Human Occludin (OCLN) ELISA Kit | OCLN elisa kit

Human Occludin (OCLN) ELISA Kit

Cat. Num. AAA2024381

• Gene Names: OCLN; BLCPMG; PPP1R115
• Reactivity: Human
• Synonyms: Occludin (OCLN); Human Occludin (OCLN) ELISA Kit; Tight Junction Protein Occludin TM4 Minus; OCLN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Occludin (OCLN).; No significant cross-reactivity or interference between Occludin (OCLN) and analogues was observed.
• Sequence Length: 70

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Occludin (OCLN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Occludin (OCLN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OCLN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Occludin (OCLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Occludin (OCLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Occludin (OCLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Occludin (OCLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for OCLN elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1322282
• NCBI GeneID: 100506658
• UniProt Accession #: Q16625; Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9
• Molecular Weight: 8,175 Da
• NCBI Official Full Name: occludin
• NCBI Official Synonym Full Names: occludin
• NCBI Official Symbol: OCLN
• NCBI Official Synonym Symbols: BLCPMG; PPP1R115
• NCBI Protein Information: occludin
• UniProt Protein Name: Occludin
• Protein Family: Occludin
• UniProt Gene Name: OCLN
• UniProt Entry Name: OCLN_HUMAN

NCBI Description

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Uniprot Description

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria