Bovine Xeroderma Pigmentosum, Complementation Group C ELISA Kit | XPC elisa kit

Bovine Xeroderma Pigmentosum, Complementation Group C ELISA Kit

Cat. Num. AAA756309

• Gene Names: XPC; XP3; RAD4; XPCC; p125
• Reactivity: Bovine
• Synonyms: Xeroderma Pigmentosum; Complementation Group C; Bovine Xeroderma Pigmentosum; Complementation Group C ELISA Kit; XPC elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XPC elisa kit

Principle of the assay: XPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XPC antibody and an XPC-HRP conjugate. The assay sample and buffer are incubated together with XPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XPC concentration since XPC from samples and XPC-HRP conjugate compete for the anti-XPC antibody binding site. Since the number of sites is limited, as more sites are occupied by XPC from the sample, fewer sites are left to bind XPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XPC concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16741636
• NCBI GeneID: 7508
• Molecular Weight: 15,712 Da
• NCBI Official Full Name: Xeroderma pigmentosum, complementation group C
• NCBI Official Synonym Full Names: xeroderma pigmentosum, complementation group C
• NCBI Official Symbol: XPC
• NCBI Official Synonym Symbols: XP3; RAD4; XPCC; p125
• NCBI Protein Information: DNA repair protein complementing XP-C cells; mutant xeroderma pigmentosum group C
• UniProt Protein Name: DNA repair protein complementing XP-C cells
• Protein Family: DNA repair protein
• UniProt Gene Name: XPC
• UniProt Synonym Gene Names: XPCC
• UniProt Entry Name: XPC_HUMAN

NCBI Description

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

XPC: a probable nuclear protein involved in DNA excision repair. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. Defects in XPC are a cause of xeroderma pigmentosum complementation group C.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: protein binding; damaged DNA binding; bubble DNA binding; single-stranded DNA binding; loop DNA binding

Biological Process: response to drug; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair; intra-S DNA damage checkpoint; nucleotide-excision repair, DNA damage removal; DNA repair; response to UV-B

Disease: Xeroderma Pigmentosum, Complementation Group C