Rabbit anti-Human GSDMC Polyclonal Antibody | anti-GSDMC antibody

GSDMC, ID (GSDMC, MLZE, Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor)

Cat. Num. AAA646547

• Gene Names: GSDMC; MLZE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GSDMC; Polyclonal Antibody; ID (GSDMC; MLZE; Gasdermin-C; Melanoma-derived leucine zipper-containing extranuclear factor); Anti -GSDMC; anti-GSDMC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GSDMC antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the Central region of human GSDMC.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of WiDr cell line lysates (35ug/lane) using MBS646547. This demonstrates MBS646547 detected the GSDMC protein (arrow).)

Testing Data

(All lanes: MBS646547 (1:2000) Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate. Lysates/proteins are at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 58kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS646547 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646547 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of WiDr cells using MBS646547 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GSDMC antibody

Antibodies; Abs to Leucine Zipper Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13899221
• NCBI GeneID: 56169
• NCBI Accession #: NP_113603.1
• NCBI GenBank Nucleotide #: NM_031415.2
• UniProt Accession #: Q9BYG8; Q5XKF3; Q6P494
• Molecular Weight: 57,692 Da
• NCBI Official Full Name: gasdermin-C
• NCBI Official Synonym Full Names: gasdermin C
• NCBI Official Symbol: GSDMC
• NCBI Official Synonym Symbols: MLZE
• NCBI Protein Information: gasdermin-C; melanoma-derived leucine zipper, extra-nuclear factor; melanoma-derived leucine zipper-containing extranuclear factor
• UniProt Protein Name: Gasdermin-C
• Protein Family: Gasdermin
• UniProt Gene Name: GSDMC
• UniProt Synonym Gene Names: MLZE
• UniProt Entry Name: GSDMC_HUMAN

Uniprot Description

Subcellular location: Cytoplasm Ref.1.

Tissue specificity: Expressed primarily in trachea and spleen. Ref.1

Sequence similarities: Belongs to the gasdermin family.

Caution: Despite its name, it is unclear if this protein contains a functional leucine zipper.