Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit | XPC elisa kit

Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit

Cat. Num. AAA2024351

• Gene Names: XPC; XP3; RAD4; XPCC; p125
• Reactivity: Human
• Synonyms: Xeroderma Pigmentosum; Complementation Group C (XPC); Human Xeroderma Pigmentosum; Complementation Group C (XPC) ELISA Kit; XP-C; XP3; XPCC; RAD4; Xeroderma Pigmentosum Group C Protein; DNA repair protein complementing XP-C cells; XPC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group C (XPC).; No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group C (XPC) and analogues was observed.
• Sequence Length: 140

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group C (XPC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group C (XPC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XPC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group C (XPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group C (XPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for XPC elisa kit

Metabolic pathway; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 16741636
• NCBI GeneID: 7508
• UniProt Accession #: Q01831; Q53GT7; Q96AX0; B4DIP3; E9PB96; E9PH69
• Molecular Weight: 15,712 Da
• NCBI Official Full Name: Xeroderma pigmentosum, complementation group C
• NCBI Official Synonym Full Names: xeroderma pigmentosum, complementation group C
• NCBI Official Symbol: XPC
• NCBI Official Synonym Symbols: XP3; RAD4; XPCC; p125
• NCBI Protein Information: DNA repair protein complementing XP-C cells
• UniProt Protein Name: DNA repair protein complementing XP-C cells
• Protein Family: DNA repair protein
• UniProt Gene Name: XPC
• UniProt Synonym Gene Names: XPCC
• UniProt Entry Name: XPC_HUMAN

NCBI Description

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

XPC: a probable nuclear protein involved in DNA excision repair. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. Defects in XPC are a cause of xeroderma pigmentosum complementation group C.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: protein binding; damaged DNA binding; bubble DNA binding; single-stranded DNA binding; loop DNA binding

Biological Process: response to drug; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair; intra-S DNA damage checkpoint; nucleotide-excision repair, DNA damage removal; DNA repair; response to UV-B

Disease: Xeroderma Pigmentosum, Complementation Group C