WWTR1 recombinant protein

WWTR1, 1-400aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205134

• Gene Names: WWTR1; TAZ
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: WWTR1; 1-400aa; Human; His tag; E Coli (Denatured); WW domain containing transcription regulator 1; TAZ; WWTR1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL
• Sequence Length: 400

• Applicable Applications for WWTR1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for WWTR1 recombinant protein

WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for WWTR1 recombinant protein

Stem Cell

References

Huang,W., et al. (2012) J. Biol. Chem. 287 (31), 26245-26253; Pan,J., et al. (2012) Oncol. Rep. 28 (1), 179-185;

NCBI and Uniprot Product Information

• NCBI GI #: 270132744
• NCBI GeneID: 25937
• NCBI Accession #: NP_001161752
• NCBI GenBank Nucleotide #: NM_001168280.2
• UniProt Accession #: Q9GZV5; Q8N3P2; Q9Y3W6; D3DNH7
• Molecular Weight: 46.5kDa (423aa)
• NCBI Official Full Name: WW domain-containing transcription regulator protein 1
• NCBI Official Synonym Full Names: WW domain containing transcription regulator 1
• NCBI Official Symbol: WWTR1
• NCBI Official Synonym Symbols: TAZ
• NCBI Protein Information: WW domain-containing transcription regulator protein 1
• UniProt Protein Name: WW domain-containing transcription regulator protein 1
• Protein Family: WW domain-containing transcription regulator protein
• UniProt Gene Name: WWTR1
• UniProt Synonym Gene Names: TAZ

Uniprot Description

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.