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Dot Blot (DB) (Dot Blot analysis of MBS6004219 on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug/ml.)

Rabbit anti-Human SNAP25 Polyclonal Antibody | anti-SNAP25 antibody

SNAP25, Phosphorylated (pT138) (SNAP25, SNAP, Synaptosomal-associated protein 25, Super protein, Synaptosomal-associated 25kD protein) (MaxLight 750)

Gene Names
SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Reactivity
Human
Applications
Dot Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
SNAP25; Polyclonal Antibody; Phosphorylated (pT138) (SNAP25; SNAP; Synaptosomal-associated protein 25; Super protein; Synaptosomal-associated 25kD protein) (MaxLight 750); Synaptosomal-associated 25kD protein; anti-SNAP25 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SNAP25.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-SNAP25 antibody
Dot Blot (DB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide corresponding to amino acid residues surrounding T138 of human SNAP25, UniProt Accession #P60880. Species sequence homology: zebrafish, bovine, chicken, mouse and rat
Conjugate
MaxLight750
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot Blot analysis of MBS6004219 on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug/ml.)

Dot Blot (DB) (Dot Blot analysis of MBS6004219 on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug/ml.)
Related Product Information for anti-SNAP25 antibody
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Product Categories/Family for anti-SNAP25 antibody
References
1. Mohrmann, R., et al. Science 330(6003):502-505(2010). 2. Tsai, Y.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(38):16554-16559(2010). 3. Condliffe, S.B., et al. J. Biol. Chem. 285(32):24968-24976(2010). 4. Weber, J.P., et al. EMBO J. 29(15):2477-2490(2010). 5. Walter, A.M., et al. J. Cell Biol. 188(3):401-413(2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,315 Da
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25A
NCBI Official Synonym Full Names
synaptosomal-associated protein, 25kDa
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
NCBI Protein Information
synaptosomal-associated protein 25; SUP; super protein; resistance to inhibitors of cholinesterase 4 homolog
UniProt Protein Name
Synaptosomal-associated protein 25
Protein Family
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP
UniProt Entry Name
SNP25_HUMAN

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: SNARE complex; presynaptic membrane; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; synaptic vesicle priming; regulation of insulin secretion; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18

Research Articles on SNAP25

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Product Notes

The SNAP25 snap25 (Catalog #AAA6349241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNAP25, Phosphorylated (pT138) (SNAP25, SNAP, Synaptosomal-associated protein 25, Super protein, Synaptosomal-associated 25kD protein) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP25 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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