Rabbit anti-Human WWTR1 Polyclonal Antibody | anti-WWTR1 antibody

WWTR1, CT (WWTR1, TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif) (Biotin)

Cat. Num. AAA6363865

• Gene Names: WWTR1; TAZ
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WWTR1; Polyclonal Antibody; CT (WWTR1; TAZ; WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif) (Biotin); Transcriptional coactivator with PDZ-binding motif; anti-WWTR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-WWTR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WWTR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the C-terminal region of human WWTR1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WWTR1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WWTR1 antibody detected the WWTR1 protein (arrow).)

Related Product Information for anti-WWTR1 antibody

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Product Categories/Family for anti-WWTR1 antibody

Antibodies; PDZ Domain

References

Remue, E., et al. FEBS Lett. 584(19):4175-4180(2010). Strakova, Z., et al. Biol. Reprod. 82(6):1112-1118(2010). Cho, H.H., et al. J. Cell. Physiol. 223(1):168-177(2010). McGeachie, M., et al. Circulation 120(24):2448-2454(2009). Ferrara, A.M., et al. J. Endocrinol. Invest. 32(3):238-241(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 270132744
• NCBI GeneID: 25937
• NCBI Accession #: NP_001161752.1; NP_056287.1
• NCBI GenBank Nucleotide #: NM_001168280.1
• UniProt Accession #: Q9GZV5; Q8N3P2; Q9Y3W6; D3DNH7
• Molecular Weight: 44,101 Da
• NCBI Official Full Name: WW domain-containing transcription regulator protein 1
• NCBI Official Synonym Full Names: WW domain containing transcription regulator 1
• NCBI Official Symbol: WWTR1
• NCBI Official Synonym Symbols: TAZ
• NCBI Protein Information: WW domain-containing transcription regulator protein 1; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ-binding motif
• UniProt Protein Name: WW domain-containing transcription regulator protein 1
• Protein Family: WW domain-containing transcription regulator protein
• UniProt Gene Name: WWTR1
• UniProt Synonym Gene Names: TAZ
• UniProt Entry Name: WWTR1_HUMAN

Uniprot Description

TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.

Protein type: Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3q23-q24

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; transcription coactivator activity; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; transcription, DNA-dependent; stem cell division; multicellular organism growth; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; glomerulus development; osteoblast differentiation; mesenchymal cell differentiation; regulation of transcription, DNA-dependent; negative regulation of protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; negative regulation of protein kinase activity; gene expression; positive regulation of transcription from RNA polymerase II promoter